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HSP27 (human), ELISA kit

Most comprehensive portfolio of heat shock protein (chaperone) products commercially available.
 
ADI-EKS-500 96 wells 635.00 USD
Do you need bulk/larger quantities?
 
  • Highly sentisitive ELISA, measuring as little as 0.39 ng/ml
  • Fast results from up to 39 samples in duplicate in less than 3 hours
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The HSP27 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis. Highly sensitive quantitative determination of Hsp27 independent of phosphorylation state
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Product Specification

Alternative Name:Heat shock protein 27
 
Sensitivity:0.39 ng/ml (range 0.39 - 25 ng/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human HSP27 in cell lysates, plasma, serum, and tissue.
 
Species reactivity:Human
 
Crossreactivity:HSP27 (100%). No reactivity with HSP25 (mouse or rat) or αB-Crystallin.
 
Use/Stability:Store all components at +4°, except standard at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, 5X Extraction reagent, Standard, Sample diluent, Wash buffer concentrate, Antibody, Antibody diluent, HRP Conjugate, Conjugate diluent, TMB Substrate, Stop solution 2
 
Scientific Background:Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-α, p70 S6K, PKD I, and PKC-δ. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.
 
UniProt ID:P04792
 
GenBank ID:L39370
 

Product Literature References

COPD and levels of Hsp70 (HSPA1A) and Hsp27 (HSPB1) in plasma and lymphocytes among coal workers: a case-control study: X. Cui, et al.; Cell Stress Chaperones 20, 473 (2015), Application(s): EIA using human plasma, Abstract;
Increased plasma levels of heat shock protein 70 associated with subsequent clinical conversion to mild cognitive impairment in cognitively healthy elderly: S.J. Son, et al.; PLoS One 10, e0119180 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
Integrative bioinformatics analysis of genomic and proteomic approaches to understand the transcriptional regulatory program in coronary artery disease pathways: R.K. Vangala, et al.; PLoS One 8, e57193 (2013), Application(s): ELISA using human serum, Abstract; Full Text
Electrophysiological correlation and prognostic impact of heat shock protein 27 in atrial fibrillation: Y.F. Hu, et al.; Circ. Arrhythm. Electrophysiol. 5, 334 (2012), Application(s): ELISA using human serum, Abstract; Full Text
Immunomodulatory effect of NSAID in geriatric patients with acute infection: effects of piroxicam on chemokine/cytokine secretion patterns and levels of heat shock proteins. A double-blind randomized controlled trial: I. Beyer, et al.; Cell Stress Chaperones 17, 255 (2012), Application(s): ELISA using human serum, Abstract; Full Text
Serum HSP27 is associated with medullary perfusion in kidney allografts: E. Marquez, et al.; J. Nephrol. 25, 1075 (2012), Application(s): EIA using human blood, urine, Abstract; Full Text
Effects on muscle performance of NSAID treatment with piroxicam versus placebo in geriatric patients with acute infection-induced inflammation. A double blind randomized controlled trial: I. Beyer, et al.; BMC Musculoskelet. Disord. 12, 292 (2011), Application(s): ELISA using human serum, Abstract; Full Text
The small heat shock protein 27 is a key regulator of CD8+ CD57+ lymphocyte survival: K. Wood, et al.; J. Immunol. 15, 184 (2010), Application(s): EIA using human lymphocytes, Abstract; Full Text
Heat shock proteins HSP70 and HSP27 in the cerebral spinal fluid of patients undergoing thoracic aneurysm repair correlate with the probability of postoperative paralysis: J. Hecker, et al.; Cell Stress Chaperones 13, 435 (2008), Application(s): EIA using human CSF , Abstract; Full Text
BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction: A. Djamali, et al.; Am. J. Physiol. Renal Physiol. 292, F513 (2007), Application(s): EIA using human serum and urine, Abstract; Full Text
Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans: G. Paulsen, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R844 (2007), Application(s): EIA using human vastus lateralis muscle tissue, Abstract; Full Text
Differential proteome analysis of tonsils from children with chronic tonsillitis or with hyperplasia reveals disease-associated protein expression differences: T. Just, et al.; Anal. Bioanal. Chem. 384, 1134 (2006), Application(s): EIA using human tonsil protein extracts, Abstract;

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