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p70 S6K activity kit

 
ADI-EKS-470 96 wells 591.00 USD
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  • Safe - non-radioactive measurement of kinase activity
  • Flexible - kinetic and end-point options available
  • Fast - results in < 4.5 hours
  • Efficient - only 30 μl diluted sample needed per well
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Specification

Applications:Colorimetric detection
Activity assay
 
Application Notes:For the measurement of p70 S6K activity in partially purified, purified, or crude enzyme preparations from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°, except active kinase at -80°.
 
Shipping:Shipped on Dry Ice
 
Kit/Set Contains:Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
 
Scientific Background:p70 S6K is a mitogen stimulated serine/threonine protein kinase that regulates the cell growth and G1 phase cell cycle progression. p70 S6K is activated by serum stimulation and can be inhibited by both wortmannin and rapamycin. Activation of p70 S6K by multi-site phosphorylation requires phosphoinositide 3-kinase (PI3-K)-dependent signal(s) leading to sequential phosphorylation at two key residues: Thr389 and Thr229. Thr229 phosphorylation is mediated by phosphoinositide-dependent kinase 1 (PDK-1), and is dependent upon prior phosphorylation of Thr389. Multiple residues in the p70 S6K pseudosubstrate domain (e.g. Ser411, Thr421, and Ser424) are also subject to activating phosphorylation by upstream kinases.
 
UniProt ID:P23443 (S6K1), Q9UBS0 (S6K2)
 

Product Literature References

Ribosomal protein S6 kinase1 coordinates with TOR-Raptor2 to regulate thylakoid membrane biosynthesis in rice: L. Sun, et al.; Biochim. Biophys. Acta 1861, 639 (2016), Application(s): Analyzed S6K1 kinase activity, Abstract;
Functional role of RNA polymerase II and P70 S6 kinase in KCl withdrawal-induced cerebellar granule neuron apoptosis: J. Padmanabhan, et al. ; J. Biol. Chem. 290, 5267 (2015), Application(s): Assay, Abstract; Full Text

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