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Rac1 activation kit

ADI-EKS-450 30 tests 988.00 USD
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The Rac1 activation kit provides a simple, convenient and efficient tool for monitoring the activation of the small GTPase, Rac1. This assay uses a GST-fusion protein containing the p21-binding domain (PBD) of human p21-activated kinase 1 (Pak1) to affinity precipitate active Rac1 (GTP-Rac1) from cell lysates. The GST-Pak-PBD fusion protein (~35kDa) is incubated with cell lysate and a glutathione resin. The pulled-down active or GTP-Rac1 is detected by Western blot analysis using a specific Rac1 Antibody.
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Product Specification

Activity assay, GST pulldown
Application Notes:For the measurement of active Rac1 in cell lysates of human, mouse, rat, dog, and chicken origin.
Species reactivity:Human, Mouse, Rat
Chicken, Dog
Use/Stability:Reagents require separate storage conditions upon receipt.
Shipping:Shipped on Blue Ice
Contents:Glutathione Resin, 2X SDS Sample Buffer, 1X Lysis/Binding/Wash Buffer, Antibody, GST-Pak1-PBD, 100X GDP, 100X GTP, Spin Cups, Collection Tubes
Scientific Background:Rac, cdc42 and the Rho subfamilies belong to the Rho-family of small GTP-binding proteins (G proteins or GTPases), which have been implicated in regulating a variety of cellular functions including actin cytoskeleton organization, cell growth control and development, transcriptional activation, membrane trafficking, and cell transformation. Cdc42/Rac are important upstream regulators of the protein kinase cascade that controls the SAPK/Jnk and p38 activity. Like all small GTPases, Rac acts as a switch regulating molecular events by cycling between an inactive GDP-bound state and an active GTP-bound state.
UniProt ID:P63000

Product Literature References

PKCε-mediated c-Met endosomal processing directs fluctuant c-Met-JNK-paxillin signaling for tumor progression of HepG2: C.T. Hu, et al.; Cell. Signal. 27, 1544 (2015), Application(s): Assay, Abstract;
Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption: T.S. Pavlov, et al.; J. Biol. Chem. 289, 28651 (2014), Abstract;

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