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PKC kinase activity kit

Non-radioactive protein kinase activity assay provides rapid and sensitive detection
 
ADI-EKS-420A 96 wells 483.00 USD
Bulk Request
 
  • Non-radioactive measurement of kinase activity
  • Flexible format, kinetic and end-point options available
  • Fast results in < 4.5 hours
  • Efficiency maximized with only 30 µl diluted sample needed per well
The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Specification

Alternative Name:Protein kinase C
 
Assay Time:4.5 hours
 
Applications:Colorimetric detection
 
Application Notes:For the measurement of PKC kinase activity partially purified, purified, or crude enzyme preparations from any species. Cited sample type includes tissue (ref. 1).
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°, except active kinase at -80°.
 
Kit/Set Contains:Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
 
Scientific Background:Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC).
 

Product Literature References

Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling: P. Waghe, et al.; Chem. Biol. Interact. 237, 104 (2015), Application(s): Assay using rat aortic tissue homogenate , Abstract;
NADPH oxidase NOX1 is involved in activation of protein kinase C and premature senescence in early-stage diabetic kidney: K. Zhu, et al.; Free Radic. Biol. Med. (2015), Application(s): Assay, Abstract;
PreImplantation Factor bolsters neuroprotection via modulating Protein Kinase A and Protein Kinase C signaling: M. Mueller, et al.; Cell Death Differ. (2015), Application(s): ELISA using mouse neuroblastoma cells, Abstract; Full Text
Urea-induced ROS cause endothelial dysfunction in chronic renal failure: M. D'Apolito, et al.; Atherosclerosis 239, 393 (2015), Application(s): Assay, Abstract;
CCK8 negatively regulates the TLR9-induced activation of human peripheral blood pDCs by targeting TRAF6 signaling: X. Jia, et al.; Eur. J. Immunol. 44, 489 (2014), Abstract;
Chromatinized protein kinase C-theta: can it escape the clutches of NF-kappaB?: E.L. Sutcliffe, et al.; Front. Immun. 3, 260 (2012),
PKC-Dependent Human Monocyte Adhesion Requires AMPK and Syk Activation: M. Chang, et al.; PLoS One 7, e40999 (2012),
Reduced Activity of Protein Kinase C in the Frontal Cortex of Subjects with Regressive Autism: Relationship with Developmenal Abnormalities: J. Lina, et al.; Int. J. Biol. Sci. 8, 1075 (2012),
Anti-inflammatory actiity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress: A. Triantafyllou, et al.; Nutrition 10, 64 (2011),
Anti-inflammatory and antitumor promotional effects of a novel urinary metabolite, 3',4'-didemethylnobiletin, derived from nobiletin: M. Pan, et al. ; Carcinogenesis 29, 2415 (2008), Application(s): EIA using mouse tissue (ref. 1), Abstract;

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