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PKC kinase activity kit

 
ADI-EKS-420A 96 wells 469.00 USD
 
  • Safe - non-radioactive measurement of kinase activity
  • Flexible - kinetic and end-point options available
  • Fast - results in < 4.5 hours
  • Efficient - only 30 µl diluted sample needed per well
The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Specification

Application:For the measurement of PKC kinase activity partially purified, purified, or crude enzyme preparations from any species. Cited sample type includes tissue (ref. 1).
 
Use/Stability:Store all components at +4°, except active kinase at -80°.
 
Kit/Set Contains:Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
 
Miscellaneous/General:Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC).
 

Product Literature References

CCK8 negatively regulates the TLR9-induced activation of human peripheral blood pDCs by targeting TRAF6 signaling: X. Jia, et al.; Eur. J. Immunol. 44, 489 (2014), Abstract;
Chromatinized protein kinase C-theta: can it escape the clutches of NF-kappaB?: E.L. Sutcliffe, et al.; Front. Immun. 3, 260 (2012),
PKC-Dependent Human Monocyte Adhesion Requires AMPK and Syk Activation: M. Chang, et al.; PLoS One 7, e40999 (2012),
Reduced Activity of Protein Kinase C in the Frontal Cortex of Subjects with Regressive Autism: Relationship with Developmenal Abnormalities: J. Lina, et al.; Int. J. Biol. Sci. 8, 1075 (2012),
Anti-inflammatory actiity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress: A. Triantafyllou, et al.; Nutrition 10, 64 (2011),
Anti-inflammatory and antitumor promotional effects of a novel urinary metabolite, 3',4'-didemethylnobiletin, derived from nobiletin: M. Pan, et al. ; Carcinogenesis 29, 2415 (2008), Application(s): EIA using mouse tissue (ref. 1), Abstract;

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