Akt kinase activity kit



ADI-EKS-400A	96 wells	425.00 USD

Safe - non-radioactive measurement of kinase activity
Flexible - kinetic and end-point options available
Fast - results in < 4.5 hours
Efficient - only 30 �l diluted sample needed per well

This is a non-radioactive Akt kinase activity assay, providing a safe, rapid and reliable method for the screening of inhibitors or activators of Akt and for quantitating the activity of Akt in purified or partially purified enzyme preparations. The Akt Kinase activity kit is based on a solid phase enzyme immunoassay (EIA) that utilizes a specific synthetic peptide as a substrate for Akt and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of Akt (1, 2, 3) activity in the solution phase.

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Product Specification

Kit/Set Contains:	Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2

Application:	For the measurement of Akt in partially purified, purified, or crude enzyme preparations from any species.

Use/Stability:	Store all components at +4°, except active kinase at -80°.

Miscellaneous/General:	bits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3β, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.