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PKA kinase activity kit

Non-radioactive protein kinase assay provides rapid and sensitive detection
 
ADI-EKS-390A 96 wells 519.00 USD
Do you need bulk/larger quantities?
 
  • Safe and non-radioactive colorimetric measurement of kinase activity
  • Flexible format, kinetic and end-point options available
  • Fast results in < 4.5 hours
  • Efficiency maximized with only 30 µl diluted sample needed per well
The PKA Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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Product Specification

Alternative Name:Protein kinase A, cAMP-dependent protein kinase
 
Applications:Colorimetric detection
Activity assay
 
Application Notes:For the quantitative determination of PKA kinase activity in partially purified, purified, or crude enzyme preparations from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°, except active kinase at -80°.
 
Shipping:Shipped on Dry Ice
 
Kit/Set Contains:Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
 
Scientific Background:PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.
 
UniProt ID:P17612 (Cα subunit), P22694 (Cβ subunit), P22162 (Cγ subunit), P10644 (RIα subunit), P31321 (RIβ subunit), P13861 (RIIα subunit), P31323 (RIIβ subunit)
 

Product Literature References

Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells: S.N. Quinn, et al.; Mol. Carcinog. 56, 1344 (2017), Abstract;
Afzelin positively regulates melanogenesis through the p38 MAPK pathway: E. Jung, et al. ; Chem. Biol. Interact. 254, 167 (2016), Application(s): PKA kinase activity was measured in cell lysates, Abstract;
Impact of 3-Amino-1,2,4-Triazole (3-AT)-Derived Increase in Hydrogen Peroxide Levels on Inflammation and Metabolism in Human Differentiated Adipocytes: F.J. Ruiz-Ojeda et al.; PLoS One 11, e0152550 (2016), Abstract; Full Text
Linagliptin Has Wide-Ranging Anti-Inflammatory Points of Action in Human Umbilical Vein Endothelial Cells: Y. Nakamura, et al.; Jpn. Clin. Med. 7, 27 (2016), Abstract; Full Text
Protein Kinase A Activation Promotes Cancer Cell Resistance to Glucose Starvation and Anoikis: R. Palorini, et al.; PLoS One 12, e1005931 (2016), Application(s): Determination of PKA activity, Abstract;
Vasopressin/V2 receptor stimulates renin synthesis in the collecting duct: A. Gonzalez, et al.; Am. J. Physiol. Renal Physiol. 310, F284 (2016), Abstract;
Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway: F. Ando, et al.; Nat. Commun. 7, 13636 (2016), Abstract; Full Text
Activation of OR1A1 suppresses PPAR-γ expression by inducing HES-1 in cultured hepatocytes: C. Wu, et al.; Int. J. Biochem. Cell. Biol. 64, 75 (2015), Application(s): ELISA using human cells, Abstract;
Activation of PKA/CREB Signaling is Involved in BMP9-Induced Osteogenic Differentiation of Mesenchymal Stem Cells: H. Zhang, et al.; Cell. Physiol. Biochem. 37, 548 (2015), Application(s): ELISA measuring PKA in mouse samples, Abstract; Full Text
FNDC5 overexpression and irisin ameliorate glucose/lipid metabolic derangements and enhance lipolysis in obesity: X.Q. Xiong, et al.; Biochim. Biophys. Acta. 1852, 1867 (2015), Application(s): ELISA using mouse, Abstract;
Human monocyte recognition of adenosine-based cyclic dinucleotides unveils the A2a Gαs protein-coupled receptor tonic inhibition of mitochondrially induced cell death: M. Tosolini, et al.; Mol. Biol. Cell. 35, 479 (2015), Application(s): PKA kinase activity in purified human monocytes, Abstract; Full Text
In situ fabrication of cleavable peptide arrays on polydimethylsiloxane and applications for kinase activity assays: H.H. Chen, et al.; Anal. Chim. Acta 865, 53 (2015), Application(s): Assay, Abstract;
Phosphodiesterase-4D Knock-down in the Prefrontal Cortex Alleviates Chronic Unpredictable Stress-Induced Depressive-Like Behaviors and Memory Deficits in Mice: Z.Z. Wang, et al.; Sci. Rep. 5, 11332 (2015), Application(s): ELISA using mouse sample, Abstract; Full Text
PreImplantation Factor bolsters neuroprotection via modulating Protein Kinase A and Protein Kinase C signaling: M. Mueller, et al.; Cell Death Differ. 22, 2078 (2015), Application(s): ELISA using mouse neuroblastoma cells, Abstract; Full Text
Role of LRP1 and ERK and cAMP Signaling Pathways in Lactoferrin-Induced Lipolysis in Mature Rat Adipocytes: K. Ikoma-Seki, et al.; PLOS one 10, e0141378 (2015), Abstract; Full Text
Short- and long-term regulation of intestinal Na+/H+ exchange by Toll-like receptors TLR4 and TLR5: J.M. Cabral, et al.; Am. J. Physiol. Gastrointest. Liver Physiol. 309, G703 (2015), Abstract;
STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs: G. Garcia-Alvarez, et al.; Mol. Biol. Cell. 26, 1141 (2015), Application(s): PKA kinase activity in primary rat hippocampal neurons, Abstract; Full Text
Nicotine shifts the temporal activation of hippocampal protein kinase A and extracellular signal-regulated kinase 1/2 to enhance long-term, but not short-term, hippocampus-dependent memory: T. Gould, et al.; Neurobiol. Learn. Mem. 109, 151 (2014), Application(s): EIA using mouse hippocampal tissue, Abstract; Full Text
Nitrite activates protein kinase A in normoxia to mediate mitochondrial fusion and tolerance to ischaemia/reperfusion: C. Kamga, et al.; Cardiovasc. Res. 101, 57 (2014), Application(s): Assay of rat cardiomyocytes, Abstract; Full Text
Adenylyl cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A pathway activation: M. Sabbatini, et al.; J. Physiol. 591, 3693 (2013), Application(s): EIA using mouse pancreatic tissue , Abstract; Full Text
HPRT-deficiency dysregulates cAMP-PKA signaling and phosphodiesterase 10A expression: mechanistic insight and potential target for Lesch-Nyhan disease?: G.H. Guibinga, et al.; PLoS One 8, e63333 (2013), Abstract; Full Text
Protein kinase A-Iα regulates Na,K-ATPase endocytosis in alveolar epithelial cells exposed to high CO(2) concentrations: E. Lecuona, et al.; Am. J. Respir. Cell Mol. Biol. 48, 626 (2013), Abstract;
Short-and Long-Term Regulation of Intestinal Na+/H+ Exchange Activity Associated with TLR2 Receptor Activation Is Independent of Nuclear Factor-κB Signaling: J. Cabral, et al.; J. Pharmacol. Exp. Ther. 346, 453 (2013), Application(s): Assay of human T84 cells, mouse lung epithelial cells, and rat intestinal epithelial cells, Abstract; Full Text
The protein kinase A regulatory subunit R1A (Prkar1a) plays critical roles in peripheral nerve development: L. Guo, et al.; J. Neurosci. 33, 17967 (2013), Application(s): Analysis of mouse sciatic nerve cells and tissue by assay, WB, Abstract; Full Text
Up-regulation of ciliary neurotrophic factor in astrocytes by aspirin: implications for remyelination in multiple sclerosis: K.K. Modi, et al.; J. Biol. Chem. 288, 18533 (2013), Abstract;
Alzheimer's β-secretase (BACE1) regulates the cAMP/PKA/CREB pathway independently of β-amyloid: Y. Chen, et al.; J. Neurosci. 32, 11390 (2012), Abstract; Full Text
Mechanisms underlying the inhibitory effects of uroguanylin on NHE3 transport activity in renal proximal tubule: L.M. Lessa, et al.; Am. J. Physiol. Renal Physiol. 303, F1399 (2012), Abstract;
Selective β2-adrenoreceptor stimulation attenuates myocardial cell death and preserves cardiac function after ischemia-reperfusion injury: S. Bhushan, et al.; Arterioscler. Thromb. Vasc. Biol. 32, 1865 (2012), Abstract; Full Text
Limonene, a natural cyclic terpene, is an agonistic ligand for adenosine A(2A) receptors: H.M. Park, et al.; BBRC 404, 345 (2011), Abstract;
Neurotrophic effect of citrus 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone: promotion of neurite outgrowth via cAMP/PKA/CREB pathway in PC12 cells: H.C. Lai, et al.; PLoS One 6, e28280 (2011), Abstract; Full Text

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