Western Blot (1µg/ml, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~55kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 10mM sodium phosphate, 50mM sodium chloride, and 0.1mM PMSF.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
TCP-1alpha is a 60 kDa subunit of a cytosolic hetero-oligomer chaperone that is known to be involved in the folding of Actin and Tubulin. This protein is a member of the Chaperonin family, which includes E. coli GroEL, the mitochondrial Heat-shock protein Hsp60, the plastid Rubisco-subunit-binding protein, and the archaebacterial protein TF55. These Chaperonins assist the folding of proteins upon ATP hydrolysis.
Western blot analysis of human Jurkat cell lysate probed with TCP-1β mAb (PK/8/4/4i/2f).
IHC Analysis: Human spleen tissue stained with TCP-1 β, mAb (PK/8/4/4i/2f) at 10µg/ml.
IHC Analysis: Human testis tissue stained with TCP-1 β, mAb (PK/8/4/4i/2f) at 10µg/ml.
