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Actin polyclonal antibody

 
ADI-CSA-400-D 50 µg 161.00 USD
 
ADI-CSA-400-F 200 µg 345.00 USD
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Product Specification

Alternative Name:ACTA, ACTSA
 
Host:Rabbit
 
Immunogen:Bovine skeletal muscle Actin.
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~42kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Actin is one of the major proteins (~42 kDa) of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells which makes it a useful loading control in Western blotting experiments. In higher eukaryotes, there are six isoforms of actin: Three alpha-actins (α-skeletal, α-cardiac, α-smooth muscle), one β-actin (β-non-muscle) and two γ-actins (γ-smooth muscle and γ-non-muscle). Actin isoforms show >90% overall sequence homology.
 
CSA-400 WB
Western Blot Analysis of Actin pAb: Lane 1: MWM, Lane 2: Mouse Heart Tissue Extract, Lane 3: Chicken Heart Tissue Extract.
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CSA-400 WB

Product Literature References

Effect of HSP25 loss on muscle contractile function and running wheel activity in young and old mice: K.A. Huey, et al.; Front. Physiol. 4, 398 (2013), Application(s): WB using muscle samples, Abstract; Full Text
Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Application(s): WB using human cell lysates, Abstract;

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