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GAPDH monoclonal antibody (1D4)

 
ADI-CSA-335-E 100 µl 313.00 USD
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Product Specification

Alternative Name:Glyceraldehyde-3-phosphate dehydrogenase
 
Clone:1D4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Pig GADPH.
 
UniProt ID:P00355
 
Species reactivity:Human, Mouse, Rat
Chicken
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100-1:1,000)
Western Blot (1:1,000-1:5,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~36kDa by Western blot.
 
Formulation:Liquid. In concentrated culture supernatant containing 50% glycerol, 0.01% sodium azide, and 1.0mg/ml BSA.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is responsible for one of the steps in the glycolytic pathway. GAPDH is essential for cellular metabolism and is thus classified as a housekeeping protein, relatively high levels of the protein and its RNA being maintained all of the time. GAPDH is highly conserved across species.
 
CSA-335 WB
Western blot analysis of mouse sciatic nerve homogenate probed with GAPDH mAb (1D4). Image kindly provided by Dr. G. Shaw, University of Florida.
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CSA-335 WB

Product Literature References

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes: A.C. Wolfes, et al.; J. Gen Physiol. 149, 149 (2017), Abstract; Full Text
Chronic endometritis modifies decidualization in human endometrial stromal cells: D. Wu, et al.; Reprod. Biol. Endocrinol. 15, 16 (2017), Abstract; Full Text
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus: I. Hassouna, et al.; Mol. Psychiatry 21, 1752 (2016), Application(s): Immunoblot analysis, Abstract;
TNFα-induced airway smooth muscle cell proliferation depends on endothelin receptor signaling, GM-CSF and IL-6: J. Knobloch, et al.; Biochem. Pharmacol. 2952, 30173 (2016), Application(s): Western blot, Abstract;
An ELMO2-RHOG-ILK network modulates microtubule dynamics: B.C. Jackson, et al.; Mol. Biol. Cell 26, 2712 (2015), Application(s): Western Blot, Abstract; Full Text
SD-208, a Novel Protein Kinase D Inhibitor, Blocks Prostate Cancer Cell Proliferation and Tumor Growth In Vivo by Inducing G2/M Cell Cycle Arrest: M. Tandon, et al.; PLoS One 10, e0119346 (2015), Application(s): Western Blotting, Abstract; Full Text
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Carbon monoxide abrogates ischemic insult to neuronal cells via the soluble guanylate cyclase-cGMP pathway: N. Schallner, et al.; PLoS One 8, e60672 (2013), Application(s): WB using human cell lysate, Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Brain-derived antigens in lymphoid tissue of patients with acute stroke: A.M. Planas, et al.; J. Immunol. 188, 2156 (2012), Application(s): WB using human tissue extract, Abstract; Full Text
Essential role of integrin-linked kinase in regulation of phagocytosis in keratinocytes: S. Sayedyahossein, et al.; FASEB J. 26, 4218 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Potential estrogenic activity of triclosan in the uterus of immature rats and rat pituitary GH3 cells: E.M. Jung, et al.; Toxicol. Lett. 208, 142 (2012), Application(s): WB using rat tissue and cell lysate, Abstract;
Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells: Y.R. Kim, et al.; Int. J. Mol. Med. 29, 294 (2012), Application(s): WB using rat cell lysate, Abstract; Full Text
Caffeine stimulates cytochrome oxidase expression and activity in the striatum in a sexually dimorphic manner: F. Jones, et al. ; Mol. Pharmacol. 74, 673 (2008), Application(s): WB using mouse tissue, Abstract;
Hic-5/ARA55, a LIM domain-containing nuclear receptor coactivator expressed in prostate stromal cells: M.D. Heitzer, et al.; Cancer Res. 66, 7326 (2006), Application(s): WB using human cell lysate, Abstract; Full Text

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