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GAPDH monoclonal antibody (1D4)

 
ADI-CSA-335-E 100 µl 329.00 USD
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Product Specification

Alternative Name:Glyceraldehyde-3-phosphate dehydrogenase
 
Clone:1D4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Pig GADPH.
 
UniProt ID:P00355
 
Species reactivity:Human, Mouse, Rat
Chicken
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:100-1:1,000)
Western Blot (1:1,000-1:5,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~36kDa by Western blot.
 
Formulation:Liquid. In concentrated culture supernatant containing 50% glycerol, 0.01% sodium azide, and 1.0mg/ml BSA.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is responsible for one of the steps in the glycolytic pathway. GAPDH is essential for cellular metabolism and is thus classified as a housekeeping protein, relatively high levels of the protein and its RNA being maintained all of the time. GAPDH is highly conserved across species.
 
GAPDH monoclonal antibody (1D4) Western blot
Western blot analysis of mouse sciatic nerve homogenate probed with GAPDH mAb (1D4). Image kindly provided by Dr. G. Shaw, University of Florida.
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GAPDH monoclonal antibody (1D4) Western blot

Product Literature References

Genome-Scale CRISPR Screens Identify Human Pluripotency-Specific Genes: R.J. Ihry, et al.; Cell Rep. 27, 616 (2019), Abstract;
Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90: R.A. Sager, et al.; Cell Rep. 26, 1344 (2019), Abstract; Full Text
The role of Slit2 as a tumor suppressor in thyroid cancer: M.J. Jeon, et al.; Mol. Cell. Endocrinol. 483, 87 (2019), Abstract;
VPS13A is closely associated with mitochondria and is required for efficient lysosomal degradation: S. Munoz-Braceras, et al.; Dis. Model. Mech. 12, dmm036681 (2019), Abstract; Full Text
Mechanism of Peptide Binding and Cleavage by the Human Mitochondrial Peptidase Neurolysin: P.F. Teixeira, et al.; J. Mol. Biol. 430, 348 (2018), Abstract;
Protective role of integrin-linked kinase against oxidative stress and in maintenance of genomic integrity: M. Im, et al.; Oncotarget 9, 13637 (2018), Abstract; Full Text
Protein Kinase D2 Modulates Cell Cycle By Stabilizing Aurora A Kinase at Centrosomes: A. Roy, et al.; Mol. Cancer Res. 16, 1785 (2018), Abstract;
Selection of stable expressed reference genes in native and vitrified/thawed human ovarian tissue for analysis by qRT-PCR and Western blot: D.A. Nikishin, et al.; J. Assist. Reprod. Genet. 35, 1851 (2018), Abstract;
Sporadic renal angiomyolipoma in a patient with Birt-Hogg-Dubé: chaperones in pathogenesis: R.A. Sager, et al.; Oncotarget 9, 22220 (2018), Abstract; Full Text
Type 2 Diabetes Leads to Axon Initial Segment Shortening in db/db Mice: L.M. Yermakov, et al.; Front. Cell. Neurosci. 12, 146 (2018), Abstract; Full Text
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes: A.C. Wolfes, et al.; J. Gen Physiol. 149, 149 (2017), Abstract; Full Text
Altered mechanisms of protein synthesis in frontal cortex in Alzheimer disease and a mouse model: P. Garcia-Esparcia, et al.; Am. J. Neurodegener. Dis. 6, 15 (2017), Abstract; Full Text
CDH1 regulates E2F1 degradation in response to differentiation signals in keratinocytes: R.K. Singh, et al.; Oncotarget 8, 4977 (2017), Abstract; Full Text
Chronic endometritis modifies decidualization in human endometrial stromal cells: D. Wu, et al.; Reprod. Biol. Endocrinol. 15, 16 (2017), Abstract; Full Text
Quantification of autophagy flux using LC3 ELISA: S.H. Oh, et al.; Anal. Biochem. 530, 57 (2017), Abstract;
Expression profiles of heat shock protein 27 and αB-crystallin and their effects on heat-stressed rat myocardial cells in vitro and in vivo: S. Tang, et al.; Mol. Med. Rep. 13, 1633 (2016), Abstract;
Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus: I. Hassouna, et al.; Mol. Psychiatry 21, 1752 (2016), Application(s): Immunoblot analysis, Abstract;
TNFα-induced airway smooth muscle cell proliferation depends on endothelin receptor signaling, GM-CSF and IL-6: J. Knobloch, et al.; Biochem. Pharmacol. 2952, 30173 (2016), Application(s): Western blot, Abstract;
An ELMO2-RHOG-ILK network modulates microtubule dynamics: B.C. Jackson, et al.; Mol. Biol. Cell 26, 2712 (2015), Application(s): Western Blot, Abstract; Full Text
SD-208, a Novel Protein Kinase D Inhibitor, Blocks Prostate Cancer Cell Proliferation and Tumor Growth In Vivo by Inducing G2/M Cell Cycle Arrest: M. Tandon, et al.; PLoS One 10, e0119346 (2015), Application(s): Western Blotting, Abstract; Full Text
The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss: C.D. Ricketts, et al.; PLoS One 10, e0115334 (2015), Application(s): Western Blotting, Abstract; Full Text
Carbon monoxide abrogates ischemic insult to neuronal cells via the soluble guanylate cyclase-cGMP pathway: N. Schallner, et al.; PLoS One 8, e60672 (2013), Application(s): WB using human cell lysate, Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Brain-derived antigens in lymphoid tissue of patients with acute stroke: A.M. Planas, et al.; J. Immunol. 188, 2156 (2012), Application(s): WB using human tissue extract, Abstract; Full Text
Essential role of integrin-linked kinase in regulation of phagocytosis in keratinocytes: S. Sayedyahossein, et al.; FASEB J. 26, 4218 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
Potential estrogenic activity of triclosan in the uterus of immature rats and rat pituitary GH3 cells: E.M. Jung, et al.; Toxicol. Lett. 208, 142 (2012), Application(s): WB using rat tissue and cell lysate, Abstract;
Synergistic effects of octylphenol and isobutyl paraben on the expression of calbindin-D9k in GH3 rat pituitary cells: Y.R. Kim, et al.; Int. J. Mol. Med. 29, 294 (2012), Application(s): WB using rat cell lysate, Abstract; Full Text
Caffeine stimulates cytochrome oxidase expression and activity in the striatum in a sexually dimorphic manner: F. Jones, et al. ; Mol. Pharmacol. 74, 673 (2008), Application(s): WB using mouse tissue, Abstract;
Hic-5/ARA55, a LIM domain-containing nuclear receptor coactivator expressed in prostate stromal cells: M.D. Heitzer, et al.; Cancer Res. 66, 7326 (2006), Application(s): WB using human cell lysate, Abstract; Full Text

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