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EGF receptor monoclonal antibody (6F1)

 
ADI-CSA-330-E 100 µg 281.00 USD
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Product Specification

Alternative Name:EGFR, epidermal growth factor receptor
 
Clone:6F1
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant human EGF receptor (C-terminal fragment).
 
UniProt ID:P00533
 
Species reactivity:Human
 
Applications:Flow Cytometry, ICC, IP, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml per 5x105 cells)
Immunocytochemistry (10µg/ml)
Immunoprecipitation (10-100µg, cell extract)
Western Blot (1-5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~170kDa by Western blot.
 
Purity Detail:Ammonium sulfate precipitation purified.
 
Formulation:Lyophilized. In PBS, pH 7.2, containing 10mg/ml sucrose and 1mg/ml sodium azide. Reconstitute in 1 ml of distilled water.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The epidermal growth factor receptor (EGFR) is a 170-kDa membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region, and a tyrosine kinase domain-containing cytoplasmic region. Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules. Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g. SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation.
 
CSA-330 WB
Western blot analysis of human A431 cell lysate probed with EGFR mAb (6F1).
CSA-330 IP
Immunoprecipitation analysis of human EGF -R from A431 cells with 2µg (1) and 10μg (2) of EGFR mAb (6F1), and mouse IgG (3).
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CSA-330 WB CSA-330 IP

Product Literature References

Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells: H. Nishi, et al.; Eur. J. Pharmacol. 748, 133 (2015), Application(s): Western blot, Abstract;
Phosphorylation of epidermal growth factor receptor at serine 1047 by MAP kinase-activated protein kinase-2 in cultured lung epithelial cells treated with flagellin: N. Noguchi, et al.; Arch. Biochem. Biophys. 529, 75 (2013), Application(s): Western blot, Abstract;
Selective cleavage of ErbB4 by G-protein-coupled gonadotropin-releasing hormone receptor in cultured hypothalamic neurons: S. Higa-Nakamine, et al.; J. Cell. Physiol. 227, 2492 (2012), Abstract;

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