Annexin V-FITC kit



ADI-ADK-700-E	100 tests	335.00 USD

ADI-ADK-700-F	200 tests	650.00 USD

Annexin V is a phospholipid binding protein that belongs to the Annexin family. In the presence of calcium ions it exhibits a high affinity for binding selectively to phosphatidylserine (PS). The appearance of PS on the cell surface is one of the early structural changes in cells undergoing apoptosis. Annexin V displays very low affinity for other phospholipid species like phosphatidylethanolamine, sphingomyelin and phosphatidyl-choline, making it an ideal reagent for detecting apoptosis.

Product Specification

Kit/Set Contains:	Concentrated binding buffer, Annexin V-FITC Solution, Red solid propidium iodide

Application:	For the measurement of Annexin-V in cells via flow cytometry.

Long Term Storage:	+4°C

Miscellaneous/General:	Insights in the apoptotic process have introduced parameters to detect and measure apoptosis. One of these addresses the measurement of phosphatidylserine (PS). During apoptosis, a cell changes the structure of its plasma membrane (PM) to signal its suicide to the environment. Phagocytes pick up this signal and remove the dying cell by phagocytosis. The appearance of PS at the cell surface is one of the structural changes recognized by phagocytes. Annexin V is a phospholipid binding protein belonging to the Annexin family. In the presence of calcium ions, it exhibits a high affinity for binding selectively to PS. Annexin V displays very low affinity for phospholipid species like phosphatidylethanolamine, sphingomyelin and phosphatidylcholine. This was firstly demonstrated for model membranes, and later, blood platelets, which expose PS at their cell surface under certain activating conditions. It is this phospholipid binding property which makes Annexin V a powerful and selective tool to detect apoptotic cells. A fluorescein conjugate of Annexin V is used to stain apoptotic cells by a simple and quick procedure that requires no fixation of the cells and no washing procedures. The stained cells can be measured using flow cytometry.