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Caspase-3 polyclonal antibody

ADI-AAS-103-D 50 µl 154.00 USD
ADI-AAS-103-F 200 µl 329.00 USD
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Product Specification

Immunogen:Synthetic peptide corresponding to a portion of human caspase-3 . The sequence differs from rat caspase-3 by 1 aa and from mouse by 2 aa.
UniProt ID:P42574
GenBank ID:U26943
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Recommended Dilutions/Conditions:Western Blot (ECL, 1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~36kDa by Western blot.
Formulation:Liquid. Antiserum.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or ?executioner? caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
Western Blot Analysis of Caspase-3, pAb (Prod. No. ADI-AAS-103): Lane 1:MW Marker; Lane 2: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100); Lane 3: Jurkat Cell Lysate (Prod. No. ADI-LYC-JK100); Lane 4: Jurkat + Staurosporine
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Product Literature References

Gardenin B-induced cell death in human leukemia cells involves multiple caspases but is independent of the generation of reactive oxygen species: J. Cabrera, et al.; Chem. Biol. Interact. 256, 220 (2016), Application(s): Western Blot, Abstract;
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;
Protective effect of geranylgeranylacetone against radiation-induced delayed effects on human keratinocytes: M. Isoir, et al.; Radiat. Res. 179, 232 (2013), Abstract;
Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis 12, 1183 (2007), Application(s): WB using drosophila cell lysates, Abstract;

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