Product Specification
Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human Bcl-2. |
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UniProt ID: | P10415 |
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GenBank ID: | M14745 |
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Species reactivity: | Human Monkey
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Immunoprecipitation (1:100) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~25kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Bcl-2 alpha and beta are alternatively spliced isoforms of 25 kDa and 22 kDa integral membrane proteins that typically inhibit apoptosis; however Bcl-2 can also be pro-apoptotic. Bcl-2 is found in the mitochondrial, ER and nuclear membranes, and its subcellular location appears to affect whether it is pro-apoptotic or anti-apoptotic. Bcl-2 becomes pro-apoptotic when it is either cleaved by caspase-3 or targeted to the mitochondrial membrane. When Bcl-2 is targeted to the ER membrane, it protects cells from apoptosis induced by Bax overexpression. ER membrane Bcl-2 may protect against apoptosis by preserving the integrity of the mitochondria after an apoptotic stimulus. Bcl-2 family members are characterized by at least one of four Bcl-2 homology domains (BH1-BH4). Anti-apoptotic Bcl-2 proteins contain BH1-BH4 while pro-apoptotic proteins contain either BH1-BH3 or BH3 alone (e.g., Bad, Bid). |
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Western blot analysis of monkey Vero cell lysate probed with Bcl-2 pAb.
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Product Literature References
NaCl Induced High Cationic Hydroxyethylated Cholesterol-BasedNanoparticle-Mediated Synthetic Small Interfering RNA Transfer into Prostate Carcinoma PC-3 Cells: Y. Hattori, et al. ; Biol. Pharm. Bull.
31, 2294 (2008),
Application(s): WB ,
Abstract;
Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2:Bax ratio: C. Leeuwenburgh, et al. ; Cancer Res.
62, 4592 (2002),
Application(s): EIA using rat samples,
Abstract;
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