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Bcl-2 polyclonal antibody



ADI-AAS-070-E	100 µg	253.00 USD
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Product Specification

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human Bcl-2.

UniProt ID:	P10415

GenBank ID:	M14745

Species reactivity:	Human Monkey

Applications:	IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (1:100) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~25kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Bcl-2 alpha and beta are alternatively spliced isoforms of 25 kDa and 22 kDa integral membrane proteins that typically inhibit apoptosis; however Bcl-2 can also be pro-apoptotic. Bcl-2 is found in the mitochondrial, ER and nuclear membranes, and its subcellular location appears to affect whether it is pro-apoptotic or anti-apoptotic. Bcl-2 becomes pro-apoptotic when it is either cleaved by caspase-3 or targeted to the mitochondrial membrane. When Bcl-2 is targeted to the ER membrane, it protects cells from apoptosis induced by Bax overexpression. ER membrane Bcl-2 may protect against apoptosis by preserving the integrity of the mitochondria after an apoptotic stimulus. Bcl-2 family members are characterized by at least one of four Bcl-2 homology domains (BH1-BH4). Anti-apoptotic Bcl-2 proteins contain BH1-BH4 while pro-apoptotic proteins contain either BH1-BH3 or BH3 alone (e.g., Bad, Bid).

Western blot analysis of monkey Vero cell lysate probed with Bcl-2 pAb.

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Product Literature References

NaCl Induced High Cationic Hydroxyethylated Cholesterol-BasedNanoparticle-Mediated Synthetic Small Interfering RNA Transfer into Prostate Carcinoma PC-3 Cells: Y. Hattori, et al. ; Biol. Pharm. Bull. 31, 2294 (2008), Application(s): WB , Abstract;

Doxorubicin treatment in vivo causes cytochrome C release and cardiomyocyte apoptosis, as well as increased mitochondrial efficiency, superoxide dismutase activity, and Bcl-2:Bax ratio: C. Leeuwenburgh, et al. ; Cancer Res. 62, 4592 (2002), Application(s): EIA using rat samples, Abstract;