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Bim/BOD polyclonal antibody

 
ADI-AAP-330-E 100 µg 356.00 USD
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Product Specification

Alternative Name:BimEL, BimL, BCL2-like
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the middle of human Bim/BOD.
 
UniProt ID:O43521
 
GenBank ID:AF032457, AF032458
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (20µg/ml)
Western Blot (1µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~23kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Bim/BOD is a group of three splice variants, BimEL, BimL, and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bim is a critical regulator of lymphocyte homeostasis, acts as a barrier against autoimmune disease and is necessary for the apoptotic response to certain stress signals. All isoforms of Bim contain the C-terminal Bcl-2 homology domain known as BH3. Certain apoptotic stimuli release Bim from the complex which allows it to translocate to the mitochondria and bind to Bcl-2 or its homologues, thereby promoting apoptosis.
 
AAP-330 WB
Western blot analysis of human K562 cell lysate probed with Bim/BOD, pAb.
AAP-330 IHC
Immunohistochemistry analysis of Bim in human skin cancer cells with Bim antibody at 20µg/ml.
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AAP-330 WB AAP-330 IHC

Product Literature References

Antigen-affinity controls pre-germinal centser B cell selection by promoting Mcl-1 induction through BAFF receptor signaling: F.M. Wensveen, et al.; Sci. Rep. 6, 35673 (2016), Application(s): Immunoblot, mouse cells, Abstract; Full Text
Interaction of ARC and Daxx: A Novel Endogenous Target to Preserve Motor Function and Cell Loss after Focal Brain Ischemia in Mice: S. Donath, et al.; J. Neurosci. 36, 8132 (2016), Application(s): Immunoblotting and immunoprecipitation, Abstract; Full Text
An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo: B.J. Aubrey, et al.; Cell Rep. 10, 1422 (2015), Application(s): Western Blotting, Abstract;
Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med. 12, 2482 (2008), Application(s): WB using rat cell lysates, Abstract;
An inhibitor of c-Jun NH2-terminal kinase, SP600125, protects mice from D-galactosamine/lipopolysaccharide-induced hepatic failure by modulating BH3-only proteins: M. Takamura, et al. ; Life Sci. 80, 1335 (2007), Application(s): WB using mouse tissue, Abstract;
TRAIL/bortezomib cotreatment is potentially hepatotoxic but induces cancer-specific apoptosis within a therapeutic window: R. Koschny, et al.; Hepatology 45, 649 (2007), Abstract;
Induction of SM-20 in PC12 cells leads to increased cytochrome c levels, accumulation of cytochrome c in the cytosol, and caspase-dependent cell death: R.S. Freeman, et al. ; J. Neurochem. 85, 318 (2003), Application(s): WB using rat samples, Abstract;
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis: E.M. Johnson, et al. ; Neuron 29, 615 (2001), Application(s): WB using human samples, Abstract;

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