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Apaf-1 polyclonal antibody

ADI-AAP-300-E 100 µg 363.00 USD
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Product Specification

Alternative Name:APIP
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human Apaf-1.
UniProt ID:O14727
GenBank ID:AF013263
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (1µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~120kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:-20°C
Scientific Background:Apaf-1 (Apoptosis protease-activating factor 1), a mammalian protein homologous to C. elegans CED-4, is an essential adaptor molecule and a key linker of the mitochondrial apoptotic pathway. Apaf-1 binds to Cytochrome c (Apaf-2) leading to the formation of a large multimeric complex. This complex recruits (through CARD) Procaspase-9 and once activated, caspase-9 (Apaf-3) dissociates. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4, caspase-8 and Diva. Apaf-1 is ubiquitously expressed in human tissues.
AAP-300 WB
Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1µg/ml in the (A) absence and (B) presence of blocking peptide.
Immunohistochemistry analysis of Apaf1 in human heart tissue with Apaf1 antibody at 1µg/ml.
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AAP-300 WB AAP-300 IHC

Product Literature References

Y Nerve growth factor blocks thapsigargin-induced apoptosis at the level of the mitochondrion via regulation of Bim: A. Samali, et al. ; J. Cell. Mol. Med. 12, 2482 (2008), Application(s): WB using rat cell lysates, Abstract;
Death in the balance: alternative participation of the caspase-2 and -9 pathways in neuronal death induced by nerve growth factor deprivation: M.L. Shelanski, et al. ; J. Neurosci. 21, 5007 (2001), Application(s): WB using mouse samples, Abstract;
The adapter protein apoptotic protease-activating factor-1 (Apaf-1) is proteolytically processed during apoptosis: S. Wesselborg, et al. ; J. Biol. Chem. 276, 29772 (2001), Application(s): WB using human samples, Abstract;

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