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Mcl-1 polyclonal antibody

 
ADI-AAP-240-D 50 µg 215.00 USD
 
ADI-AAP-240-F 200 µg 389.00 USD
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Product Specification

Alternative Name:Bcl-2-like protein 3
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human Mcl-1.
 
UniProt ID:Q07820
 
GenBank ID:L08246
 
Species reactivity:Human
Dog, Guinea pig, Monkey, Porcine, Rabbit
 
Applications:IF, IHC (PS), WB
 
Recommended Dilutions/Conditions: Immunohistochemistry (paraffin sections, 1:50)
Western Blot (0.2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~42/43kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Mcl-1 is a member of the Bcl-2 family that possesses anti-apoptotic activity. Mcl-1 is an immediate-early gene activated by the GMCSF as well as IL-3 signaling pathways and plays an important role in the viability response of these cytokines. Similar to all Bcl-2 family members, Mcl-1 contains BH1, BH2, and BH3 domains; however, unlike other members of this family, Mcl-1 has an extended N-terminal domain rich in PEST sequences, which probably accounts for the short half-life of this protein.
 
AAP-240 WB
Western blot analysis of Mcl-1, pAb: Lane 1: MW marker, Lane 2: HeLa, Lane 3: 3T3, Lane 4: PC-12, Lane 5: MDCK, Lane 6: RK-13.
AAP-240 IF
Immunofluorescent analysis (confocal) staining of HeLa cells using Mcl-1 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
AAP-240 IHC
Immunohistochemistry analysis of human breast cancer tissue with Mcl-1 pAb.
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AAP-240 WB AAP-240 IF AAP-240 IHC

Product Literature References

Critical role of mitochondria-mediated apoptosis for JNJ-26481585-induced antitumor activity in rhabdomyosarcoma: U. Heinicke, et al.; Oncogene 35, 3729 (2016), Application(s): Western Blot, Abstract;
Low expression of pro-apoptotic Bcl-2 family proteins sets the apoptotic threshold in Waldenström macroglobulinemia: B.T. Gaudette, et al.; Oncogene 35, 479 (2016), Application(s): Western Blot, Abstract;
Identification of synthetic lethality of PLK1 inhibition and microtubule-destabilizing drugs: M. Hugle, et al.; Cell Death Differ. 22, 1946 (2015), Application(s): Western Blot, Abstract;

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