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Fas polyclonal antibody

 
ADI-AAP-221-D 50 µg 194.00 USD
 
ADI-AAP-221-F 200 µg 378.00 USD
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Product Specification

Alternative Name:CD95, APO-1, TNFRSF 6
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of mouse Fas.
 
UniProt ID:P25446
 
GenBank ID:M83649
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~45kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE.  Fas belongs to the tumor necrosis factor receptor (TNFR) family, which includes TNFR1, TNFR2, CD27, CD30 and CD40.  Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells.  Both Fas and TNFR contain a 70 amino acid cytoplasmic “death domain” responsible for transmitting signals for apoptosis.  Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long forms of FLIP. Insertion of an early transposable element in intron 2 of the Fas gene, as in the mouse lpr mutations, causes lymphoproliferation and autoimmunity in mutant mice, suggesting the involvement of Fas in clonal deletion of autoreactive T cells.
 
AAP-221 IHC
Immunohistochemical staining of human hepatoma tissue with Fas pAb.
AAP-221 WB
Western Blot Analysis of Fas pAb : Lane 1: Rat Brain Tissue Extract, Lane 2: Mouse Brain Tissue Extract.
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AAP-221 IHC AAP-221 WB

Product Literature References

Key apoptotic pathways for heat-induced programmed germ cell death in the testis: R.S. Swerdloff, et al. ; Endocrinology 144, 3167 (2003), Application(s): WB using rat samples, Abstract;

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