Western Blot (1:5,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~14kDa by Western blot.
May be used in immunofluorescence applications with human and rat cells.
Purity Detail:
Affinity purified.
Formulation:
Liquid. In PBS containing 0.08% sodium azide.
Shipping:
Shipped on Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Cytochrome c, which is a critical component of the electron transport chain, is a component of the functional apoptosome. Upon release from the mitochondria, cytochrome c binds to the adaptor proteins Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex. This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.