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Caspase-9 polyclonal antibody

 
ADI-AAP-149-E 100 µg 258.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Recombinant human procaspase-9 (N-terminal fragment).
 
UniProt ID:P55211
 
GenBank ID:AAC50640
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Monkey, Sheep
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:500, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~46-50kDa (proform) and ~35/37kDa, ~18kDa, ~10kDa (processed) by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
 
AAP-149 WB
Western blot analysis of untreated and apoptosisinduced Jurkat cell lysates, probed with Caspase-9 pAb.
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AAP-149 WB

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