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Caspase-12 polyclonal antibody

ADI-AAP-122-E 100 µg 363.00 USD
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Product Specification

Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of mouse Caspase-12.
UniProt ID:O08736
Gene/Protein Identifier:NM_009808 (RefSeq)
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Western Blot (0.5-1.0µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~48-50kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
AAP-122 WB
Western blot analysis of caspase-12 in human (A) and mouse (B) spleen tissue lysates with caspase-12 antibody at 1µg/ml.
Immunohistochemistry analysis of caspase-12 in human heart tissue with caspase-12 antibody at 10µg/ml.
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AAP-122 WB AAP-122 IHC

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