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Caspase-3 polyclonal antibody



ADI-AAP-113-D	50 µg	143.00 USD

ADI-AAP-113-F	200 µg	345.00 USD
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Product Specification

Host:	Rabbit

Immunogen:	Recombinant human Caspase-3 protein (full length).

UniProt ID:	P42574

GenBank ID:	U26943

Species reactivity:	Human, Mouse, Rat Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Yeast

Applications:	IHC (PS), WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~36kDa by Western blot. A lower molecular weight band refers to a proteolytic cleavage product of caspase-3.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.

Immunohistochemistry analysis of human spleen tissue stained with Caspase-3, pAb at 10µg/ml.

Western blot analysis of Caspase-3 pAb: Lane 1: MW Marker, Lane 2: Jurkat (Prod. No. ADI-LYC-JK100), Lane 3: Jurkat treated with staurosporine, Lane 4: MCF-7 (negative control), Lane 5: HeLa, (cell lysate) (Prod. No. ADI-LYC-HL100), Lane 6: Rat Spleen, Lane 7: Mouse Spleen.

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Product Literature References

AEBP1 upregulation confers acquired resistance to BRAF (V600E) inhibition in melanoma: W. Hu, et al.; Cell Death Dis. 4, e914 (2013), Application(s): WB using human melanoma cell lysates, Abstract; Full Text

Characterization of stress response in human retinal epithelial cells: V. Giansanti, et al.; J. Cell. Mol. Med. 17, 103 (2013), Application(s): WB using human adult retinal pigmented epithelial (ARPE-19) cell lysate, Abstract; Full Text

Carfilzomib and ONX 0912 inhibit cell survival and tumor growth of head and neck cancer and their activities are enhanced by suppression of Mcl-1 or autophagy: Y. Zang, et al.; Clin. Cancer Res. 18, 5639 (2012), Abstract;

Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells: A.Z. Decrion-Barthod, et al. ; Anticancer Res. 30, 4049 (2010), Application(s): WB using HeLa and CaSki cell lysate, Abstract; Full Text

Elevated retina-specific expression of the small heat shock protein,αA-crystallin, is associated with photoreceptor protection inexperimental uveitis: N. Rao, et al. ; Invest. Ophthalmol. Vis. Sci. 49, 1161 (2008), Application(s): WB using mouse cell lysates & tissue, Abstract;

Inhibition of p53 protects liver tissue against endotoxin-induced apoptotic and necrotic cell death: B. Vollmar, et al. ; FASEB J. 17, 660 (2003), Application(s): WB using rat samples, Abstract;

Activation of caspase pathways during iron chelator-mediated apoptosis: S.V. Torti, et al. ; J. Biol. Chem. 277, 25568 (2002), Application(s): WB using human samples, Abstract;

Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells: C. Hao, et al. ; J. Biol. Chem. 277, 25020 (2002), Application(s): WB using human samples, Abstract;

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