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Bak polyclonal antibody

 
ADI-AAP-030-D 50 µg 194.00 USD
 
ADI-AAP-030-F 200 µg 399.00 USD
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Product Specification

Alternative Name:BCL2L7
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human Bak.
 
UniProt ID:Q16611
 
Gene/Protein Identifier:S58873 (PIR)
 
Species reactivity:Human
Hamster, Monkey, Porcine
 
Applications:IF, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Immunoprecipitation(1:100)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~30kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.
 
AAP-030 IF
Immunofluorescent analysis (confocal) staining of He La cells using Bak pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
AAP-030 IHC
Immunohistochemistry analysis of human cervical cancer tissue with Bak pAb.
ADI-AAP-030 WB
Western blot analysis of Bak (Prod. no. ADI-AAP-030). Lane 1: MW Marker; Lane 2: A431; Lane 3: CHO-K1; Lane 4: ESK-4; Lane 5: Vero cells.
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AAP-030 IF AAP-030 IHC ADI-AAP-030 WB

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