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Bak polyclonal antibody

 
ADI-AAP-030-D 50 µg 204.00 USD
 
ADI-AAP-030-F 200 µg 419.00 USD
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Product Specification

Alternative Name:BCL2L7
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human Bak.
 
UniProt ID:Q16611
 
Gene/Protein Identifier:S58873 (PIR)
 
Source:Purified from rabbit serum.
 
Species reactivity:Human
Hamster, Monkey, Porcine
 
Applications:IF, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Immunoprecipitation(1:100)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~30kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.
 
Bak polyclonal antibody Western blot
Western blot analysis of Bak (Prod. no. ADI-AAP-030). Lane 1: MW Marker; Lane 2: A431; Lane 3: CHO-K1; Lane 4: ESK-4; Lane 5: Vero cells.
Bak polyclonal antibody Immunohistochemistry
Immunohistochemistry analysis of human cervical cancer tissue with Bak pAb.
Bak polyclonal antibody Immunofluorescence
Immunofluorescent analysis (confocal) staining of He La cells using Bak pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
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Bak polyclonal antibody Western blot Bak polyclonal antibody Immunohistochemistry Bak polyclonal antibody Immunofluorescence

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