Product Specification
Alternative Name: | BCL2L7 |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to a portion of human Bak. |
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UniProt ID: | Q16611 |
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Gene/Protein Identifier: | S58873 (PIR) |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human Hamster, Monkey, Porcine
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Applications: | IF, IHC, IP, WB
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Recommended Dilutions/Conditions: | Immunohistochemistry (1:50) Immunoprecipitation(1:100) Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~30kDa by Western blot. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Shipped on Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade. |
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Western blot analysis of Bak (Prod. no. ADI-AAP-030). Lane 1: MW Marker; Lane 2: A431; Lane 3: CHO-K1; Lane 4: ESK-4; Lane 5: Vero cells.
Immunohistochemistry analysis of human cervical cancer tissue with Bak pAb.
Immunofluorescent analysis (confocal) staining of He La cells using Bak pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.
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For Research Use Only. Not for use in diagnostic procedures.