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Fas monoclonal antibody (ZB4)

 
ADI-AAM-227-E 100 µg 291.00 USD
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Product Specification

Alternative Name:CD95, APO-1, TNFRSF 6
 
Clone:ZB4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Fas.
 
UniProt ID:P25445
 
Species reactivity:Human
 
Applications:Flow Cytometry, WB, FUNC
 
Recommended Dilutions/Conditions:Flow Cytometry (5-20µg/ml)
Western Blot (5µg/ml, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~45kDa by Western blot.

Functional Application: neutralizing.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein that is a member of the Tumor necrosis factor receptor (TNF-R) family, which includes TNF-R1, TNF-R2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long form of FLIP.
 
AAM-227 WB
Western blot analysis of human Fas expression in human Fas transfected cells; Lane1: anti-human Fas (ZB4), Lane2: anti-human Fas (UB2) and Lane3: anti-human Fas (CH11).
AAM-227 FC
Flow cytometric analysis of human Fas expression on normal human lymphocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of Fas mAb (ZB4) to the cells.
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AAM-227 WB AAM-227 FC

Product Literature References

LFA-1-targeting Leukotoxin (LtxA; Leukothera®) causes lymphoma tumor regression in a humanized mouse model and requires caspase-8 and Fas to kill malignant lymphocytes: K. M. DiFranco, et al.; Leuk. Res. 39, 649 (2015), Application(s): Positive Control, Abstract;
Enhanced CD95-mediated apoptosis contributes to radiation hypersensitivity of NBS lymphoblasts: D. Sagan, et al. ; Apoptosis 12, 753 (2007), Application(s): IF, Flow using human tissue culture, Abstract;

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