Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Cytochrome c monoclonal antibody (7H8.2C12)

 
ADI-AAM-175-E 100 µg 306.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ALX-804-245

Product Specification

Alternative Name:Cyt c
 
Clone:7H8.2C12
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Horse Cytochrome c .
 
UniProt ID:P00004
 
Species reactivity:Human, Mouse, Rat
Bovine, Guinea pig, Hamster, Monkey, Rabbit, Sheep
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~14kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Cytochrome c is an electron transport protein from mitochondria.  A primary means of caspase activation by p53 is through induction of cytochrome c release from mitochondria.  Cleavage of Bid, a Bcl 2 family member, by caspase-8 produces a truncated tBid molecule which triggers mitochondria release of cytochrome c which binds to the adaptor protein Apaf-1 and procaspase-9 to form a cytosolic apoptosome complex.  This event results in activation of procaspase-9 and its subsequent cleavage and activation of caspase-3 leading to the degradation of a series of cellular substrates and subsequently cell death.
 
Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry
IHC Analysis: Human uterus tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry
IHC Analysis: Human spleen tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry
IHC Analysis: Human brain, cortex tissue stained with Cytochrome c, mAb (7H8.2C12) at 5µg/ml.
Please mouse over
Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry Cytochrome c monoclonal antibody (7H8.2C12) Immunohistochemistry

Related Products

HeLa, (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL100-F 200 µg 114.00 USD
Do you need bulk/larger quantities?
 

Brain (mouse), (tissue extract) 

Mouse brain extract., WB | Print as PDF
 
ADI-LYT-MB100-F 200 µg 114.00 USD
Do you need bulk/larger quantities?
 

HeLa (heat shocked) , (cell lysate) 

Human, WB | Print as PDF
 
ADI-LYC-HL101-F 200 µg 114.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Monitor programmed cell death from membrane to nucleus
Monitor programmed cell death from membrane to nucleus
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2019 Enzo Life Sciences, Inc.,