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Caspase-7 monoclonal antibody (7-1-11)

 
ADI-AAM-127-E 100 µg 269.00 USD
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Product Specification

Clone:7-1-11
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:Recombinant human Caspase-7 protein (full length) .
 
UniProt ID:P55210
 
Species reactivity:Human, Mouse, Rat
Dog, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Specificity:Detects the unprocessed pro-caspase-7 (p35), caspase lacking the short amino-terminal pro-domain (p32) and fully processed caspase-7 (p19) in the apototic cell extract.
 
Applications:IHC (PS), WB
 
Recommended Dilutions/Conditions:Western Blot (0.2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Immunohistochemistry (paraffin sections)
Western Blot (0.2µg/ml, ECL)
Optimal conditions must be determined individually for each application.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.1mM PMSF.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
 
ADI-AAM-127 WB
Western blot analysis of Caspase-7, mAb (7-1-11) (Prod. No. ADI-AAM-127): Lane1: MW Marker; Lane 2: Jurkat (Prod. No.ADI-LYC-JK100); Lane 3: Jurkat plus Staurosporine; Lane 4: HeLa (Prod. No. ADI-LYC-HL100); Lane 5: HeLa plus Nocodazole; Lane 6: 3T3 (Prod. No. ADI-LYC-3T100); Lane 7: PC-12 (Prod. No. ADI-LYC-PC100).
ADI-AAM-127 IHC1
IHC Analysis: Human colon tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
ADI-AAM-127 IHC2
IHC Analysis: Human placenta tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
ADI-AAM-127 IHC3
IHC Analysis: Human spleen tissue stained with Caspase-7, mAb (7-1-11) at 10µg/ml.
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ADI-AAM-127 WB ADI-AAM-127 IHC1 ADI-AAM-127 IHC2 ADI-AAM-127 IHC3

Product Literature References

A novel Hsp70 inhibitor prevents cell intoxication with the actin ADP-ribosylating Clostridium perfringens iota toxin: K. Ernst, et al.; Sci. Rep. 6, 20301 (2016), Application(s): Incubated into membranes, Abstract; Full Text
Caspase-3-dependent cleavage of Bcl-2 promotes release of cytochrome c: J.M. Hardwick, et al. ; J. Biol. Chem. 274, 21155 (1999), Application(s): WB using human samples, Abstract;

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