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Caspase-8 monoclonal antibody (5F7)

 
ADI-AAM-118-E 100 µg 313.00 USD
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Product Specification

Alternative Name:FLICE
 
Clone:5F7
 
Host:Mouse
 
Isotype:IgG2b
 
Immunogen:C-terminal fragment of recombinant human caspase-8 (amino acids 176-460).
 
UniProt ID:Q14790
 
Species reactivity:Human
 
Applications:IHC (PS), IP, WB
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:50)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~55-57kDa by Western blot. Also detects the cleaved intermediate form (p41/43) and the activated large caspase-8 subunit p18 after apoptosis induction.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Caspase-8/FADD-like ICE (FLICE), is an ~55 kDa cytosolic protein belonging to the Fas/APO-1/CD95 death-inducing signaling complex.  Although researchers have defined eight different isoforms of caspase-8 at the mRNA level (8/a-h), significant expression of only Caspase-8/a and /b occurs at the protein level in a variety of cell lines. Caspase-8 contains two death effector domains (DEDs) and exhibits sequence homology to both FADD, a signal transducer of Fas-induced apoptosis, and to the ICE/CED-3 family of cysteine proteases. Caspase-8 binds to FADD through its amino terminal DEDs and induces apoptosis when overexpressed.  Activation of Caspase-8 involves a two-step proteolysis whereby the cleavage of Caspase-8 generates a 43 kDa fragment (p43) and a 12 kDa fragment further processed to 10 kDa, then the cleavage of receptor-associated p43 yields p26 and the release of the active site containing p18.  A number of cytosolic factors inhibit the FADD-Caspase-8 containing signaling complex, including the ICE/CED-3 family inhibitor CrmA which inhibits Caspase-8 protease activity, and the short and long forms of FLIP that bind to the FADD-Caspase-8 complex. 
 
ADI-AAM-118 IHC1
Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody (prod.no. ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
ADI-AAM-118 IHC2
Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody (prod.no. ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
ADI-AAM-118 WB1
Western blot analysis of Jurkat (1), Raji (2), HeLa (3) and WR19L (4) probed with Caspase-8, mAb (5F7).
ADI-AAM-118 WB2
Western Blot analysis of Caspase-8 fragments in apoptosis induced Jurkat cells using Caspase-8, mAb (5F7) (Prod. No. ADI-AAM-118). The Jurkat cells were treated with a FAS monoclonal antibody for the indicated time
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ADI-AAM-118 IHC1 ADI-AAM-118 IHC2 ADI-AAM-118 WB1 ADI-AAM-118 WB2

Product Literature References

RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death: G. Fettweis, et al.; Biochim. Biophys. Acta 1864, 113 (2017), Application(s): Western blot, Abstract;
dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8: Y. Estornes, et al.; Cell Death Differ. 19, 1482 (2012), Application(s): WB and IP using human cell lysates, Abstract; Full Text
p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3: T. Kwok, et al. ; Mol. Cancer Ther. 6, 1054 (2007), Application(s): WB using human cell lysates, Abstract;
Silibinin sensitizes human glioma cells to TRAIL-Mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin: K. Choi, et al. ; Cancer Res. 67, 8274 (2007), Application(s): WB using human cell lysates, Abstract;
Death-receptor activation halts clathrin-dependent endocytosis: C.D. Austin, et al.; PNAS 103, 10283 (2006), Abstract; Full Text
Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand: E. Varfolomeev, et al.; J. Biol. Chem. 280, 40599 (2005), Abstract; Full Text
Synthetic Triterpenoids Cooperate with Tumor Necrosis Factor–Related Apoptosis-Inducing Ligand to Induce Apoptosis of Breast Cancer Cells: M.L. Hyer, et al.; Cancer Res. 65, 4799 (2005), Abstract; Full Text
Interchain Proteolysis, in the Absence of a Dimerization Stimulus, Can Initiate Apoptosis-associated Caspase-8 Activation: B.M. Murphy, et al.; J. Biol. Chem. 279, 36916 (2004), Abstract; Full Text
Caspase-Dependent Cleavage of c-Abl Contributes to Apoptosis: D. Barilà, et al.; Mol. Cell. Biol. 23, 2790 (2003), Abstract; Full Text
Cephalostatin 1 Selectively Triggers the Release of Smac/DIABLO and Subsequent Apoptosis That Is Characterized by an Increased Density of the Mitochondrial Matrix: V.M. Dirsch, et al.; Cancer Res. 63, 8869 (2003), Abstract; Full Text
Role of Murine Cytomegalovirus US22 Gene Family Members in Replication in Macrophages: C. Ménard, et al.; J. Virol. 77, 5557 (2003), Abstract; Full Text
Multiple Pathways of TWEAK-Induced Cell Death: M. Nakayama, et al.; J. Immunol. 168, 734 (2002), Abstract; Full Text
A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation: A. Skaletskaya, et al.; PNAS 98, 7829 (2001), Abstract; Full Text
Matrix Attachment Regulates FAS-Induced Apoptosis in Endothelial Cells: A Role for C-Flip and Implications for Anoikis: F. Aoudjit & K. Vuori; J. Cell Biol. 152, 633 (2001), Abstract; Full Text
A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2: V.S. Goldmacher, et al.; PNAS 96, 12536 (1999), Abstract; Full Text
ATP-dependent Steps in Apoptotic Signal Transduction: Y. Eguchi, et al.; Cancer Res. 59, 2174 (1999), Abstract; Full Text
Inhibition of Fas-induced apoptosis by Bcl-2: S. Nagata, et al. ; Oncogene 17, 2549 (1998), Application(s): IP using human samples, Abstract;

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