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Caspase-8 monoclonal antibody (5F7)

ADI-AAM-118-E 100 µg 313.00 USD
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Product Specification

Alternative Name:FLICE
Immunogen:C-terminal fragment of recombinant human caspase-8 (amino acids 176-460).
UniProt ID:Q14790
Species reactivity:Human
Applications:IHC (PS), IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (1:50)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~55-57kDa by Western blot. Also detects the cleaved intermediate form (p41/43) and the activated large caspase-8 subunit p18 after apoptosis induction.
Purity Detail:Protein A-affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Caspase-8/FADD-like ICE (FLICE), is an ~55 kDa cytosolic protein belonging to the Fas/APO-1/CD95 death-inducing signaling complex.  Although researchers have defined eight different isoforms of caspase-8 at the mRNA level (8/a-h), significant expression of only Caspase-8/a and /b occurs at the protein level in a variety of cell lines. Caspase-8 contains two death effector domains (DEDs) and exhibits sequence homology to both FADD, a signal transducer of Fas-induced apoptosis, and to the ICE/CED-3 family of cysteine proteases. Caspase-8 binds to FADD through its amino terminal DEDs and induces apoptosis when overexpressed.  Activation of Caspase-8 involves a two-step proteolysis whereby the cleavage of Caspase-8 generates a 43 kDa fragment (p43) and a 12 kDa fragment further processed to 10 kDa, then the cleavage of receptor-associated p43 yields p26 and the release of the active site containing p18.  A number of cytosolic factors inhibit the FADD-Caspase-8 containing signaling complex, including the ICE/CED-3 family inhibitor CrmA which inhibits Caspase-8 protease activity, and the short and long forms of FLIP that bind to the FADD-Caspase-8 complex. 
Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody ( ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody ( ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
Western blot analysis of Jurkat (1), Raji (2), HeLa (3) and WR19L (4) probed with Caspase-8, mAb (5F7).
Western Blot analysis of Caspase-8 fragments in apoptosis induced Jurkat cells using Caspase-8, mAb (5F7) (Prod. No. ADI-AAM-118). The Jurkat cells were treated with a FAS monoclonal antibody for the indicated time
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Product Literature References

RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death: G. Fettweis, et al.; Biochim. Biophys. Acta 1864, 113 (2017), Application(s): Western blot, Abstract;
dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8: Y. Estornes, et al.; Cell Death Differ. 19, 1482 (2012), Application(s): WB and IP using human cell lysates, Abstract; Full Text
p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3: T. Kwok, et al. ; Mol. Cancer Ther. 6, 1054 (2007), Application(s): WB using human cell lysates, Abstract;
Silibinin sensitizes human glioma cells to TRAIL-Mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin: K. Choi, et al. ; Cancer Res. 67, 8274 (2007), Application(s): WB using human cell lysates, Abstract;
Death-receptor activation halts clathrin-dependent endocytosis: C.D. Austin, et al.; PNAS 103, 10283 (2006), Abstract; Full Text
Molecular Determinants of Kinase Pathway Activation by Apo2 Ligand/Tumor Necrosis Factor-related Apoptosis-inducing Ligand: E. Varfolomeev, et al.; J. Biol. Chem. 280, 40599 (2005), Abstract; Full Text
Synthetic Triterpenoids Cooperate with Tumor Necrosis Factor–Related Apoptosis-Inducing Ligand to Induce Apoptosis of Breast Cancer Cells: M.L. Hyer, et al.; Cancer Res. 65, 4799 (2005), Abstract; Full Text
Interchain Proteolysis, in the Absence of a Dimerization Stimulus, Can Initiate Apoptosis-associated Caspase-8 Activation: B.M. Murphy, et al.; J. Biol. Chem. 279, 36916 (2004), Abstract; Full Text
Caspase-Dependent Cleavage of c-Abl Contributes to Apoptosis: D. Barilà, et al.; Mol. Cell. Biol. 23, 2790 (2003), Abstract; Full Text
Cephalostatin 1 Selectively Triggers the Release of Smac/DIABLO and Subsequent Apoptosis That Is Characterized by an Increased Density of the Mitochondrial Matrix: V.M. Dirsch, et al.; Cancer Res. 63, 8869 (2003), Abstract; Full Text
Role of Murine Cytomegalovirus US22 Gene Family Members in Replication in Macrophages: C. Ménard, et al.; J. Virol. 77, 5557 (2003), Abstract; Full Text
Multiple Pathways of TWEAK-Induced Cell Death: M. Nakayama, et al.; J. Immunol. 168, 734 (2002), Abstract; Full Text
A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation: A. Skaletskaya, et al.; PNAS 98, 7829 (2001), Abstract; Full Text
Matrix Attachment Regulates FAS-Induced Apoptosis in Endothelial Cells: A Role for C-Flip and Implications for Anoikis: F. Aoudjit & K. Vuori; J. Cell Biol. 152, 633 (2001), Abstract; Full Text
A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2: V.S. Goldmacher, et al.; PNAS 96, 12536 (1999), Abstract; Full Text
ATP-dependent Steps in Apoptotic Signal Transduction: Y. Eguchi, et al.; Cancer Res. 59, 2174 (1999), Abstract; Full Text
Inhibition of Fas-induced apoptosis by Bcl-2: S. Nagata, et al. ; Oncogene 17, 2549 (1998), Application(s): IP using human samples, Abstract;

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