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Bcl-2 monoclonal antibody (83-8B)

 
ADI-AAM-072-E 100 µg 313.00 USD
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Product Specification

Clone:83-8B
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant rat Bcl-2 β.
 
UniProt ID:P49950
 
Gene/Protein Identifier:NP_058689 (RefSeq)
 
Species reactivity:Human, Mouse, Rat
Drosophila
 
Applications:Flow Cytometry, WB
 
Recommended Dilutions/Conditions:Flow Cytometry (10µg/ml)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~26kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Bcl-2 family of proteins regulate the intrinsic apoptotic pathway and are characterized by the sharing of at least one of four conserved Bcl-2 Homology (BH) domains. Bcl-2 family members function as either pro- or anti-apoptotic factors, and are generally categorized into three groups based on structural homology and function. Bcl-2-like proteins (Bcl-2, Bcl-xL, Bcl-w, A1, and Mcl-1) are pro-survival. Pro-apoptotic members can be sub-divided into two groups: the multi-BH domain group, including Bax, Bak, and Bok; and the more numerous and diverse BH3-only group, which includes Bim, Bad, and Bid.
 
AAM-072 WB
Western blot analysis of Jurkat (1), Raji (2), WR19L (3), and PC12 (4) cell lysates , probed with Bcl-2 mAb (83-8B).
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AAM-072 WB

Product Literature References

Rapid cold-hardening protects Drosophila melanogaster from cold-induced apoptosis: R.E. Lee, et al. ; Apoptosis 12, 1183 (2007), Application(s): WB using drosophila cell lysates, Abstract;

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