Product Details
| Alternative Name: | X-chromosome-linked inhibitor of apoptosis protein |
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| Clone: | 2F1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Recombinant human XIAP. |
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| UniProt ID: | P98170 |
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| GenBank ID: | AAC50373 |
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| Species reactivity: | Human, Mouse
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| Applications: | IHC, IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~55kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | XIAP (X-linked inhibitor of apoptosis) belongs to the IAP (inhibitors of apoptosis) family, which is characterized by one or more BIR (baculovirus IAP repeat) domains. These proteins have multiple biological functions that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. XIAP functions by direct binding and inhibition of several caspases, including caspase-3, caspase-7 and caspase-9. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Jurkat (1), Raji (2), HeLa (3), WR19L (4) and PC12 (5) cells, probed with XIAP mAb (2F1).
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Product Literature References
A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods
15, 909 (2018),
Abstract;
Heat Shock Protein 70 (Hsp70) Suppresses RIP1-Dependent Apoptotic and Necroptotic Cascades: S.R. Srinivasan, et al.; Mol. Cancer Res.
16, 58 (2018),
Abstract;
Full Text
X-linked inhibitor of apoptosis protein (XIAP) is a client of heat shock protein 70 (Hsp70) and a biomarker of its inhibition: L.C. Cesa, et al.; J. Biol. Chem.
293, 2370 (2018),
Abstract;
Full Text
The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells: C. Krepler, et al.; Clin. Cancer Res.
19, 1784 (2013),
Abstract;
Full Text
Silibinin sensitizes human glioma cells to TRAIL-Mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin: K. Choi, et al. ; Cancer Res.
67, 8274 (2007),
Application(s): WB using human cell lysates,
Abstract;
Interaction between XIAP and Smac/DIABLO in the mouse brain after transient focal cerebral ischemia: P.H. Chan, et al. ; J. Cereb. Blood Flow Metab.
23, 1010 (2003),
Application(s): IHC using mouse samples,
Abstract;
Role of Smac in human leukaemic cell apoptosis and proliferation: A.C. Newland, et al. ; Oncogene
22, 1589 (2003),
Application(s): IP using human samples,
Abstract;
XIAP, a cellular member of the inhibitor of apoptosis protein family, links the receptors to TAB1-TAK1 in the BMP signaling pathway: K. Matsumoto, et al. ; EMBO J.
18, 179 (1999),
Application(s): IP using monkey samples,
Abstract;
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