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IMMUNOSET® HO-1 (rat), ELISA development set

 
ADI-960-810 5x96 wells 483.00 USD
Do you need bulk/larger quantities?
 
  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 39 pg/mL HO-1 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents using rat cell lysates, plasma, serum, and tissue
The IMMUNOSET® HO-1 (rat), EIA development set contains sufficient reagents for the development of 5 x 96 well EIA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own EIA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
960-810-Antibody
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960-810-Antibody

Product Specification

Alternative Name:Heme oxygenase 1
 
Sensitivity:39 pg/ml (range 0.195 - 12.5 ng/ml)
 
Assay Time:Plate Coating - Overnight + 1 hour; Assay - 3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of rat HO-1 in cell lysates, plasma, serum, and tissue. Cited sample type includes bronchoalveolar lavage fluid.
 
Species reactivity:Rat
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Capture antibody, Standard, Detection antibody, SA-HRP
 
Scientific Background:Heme oxygenase (Hsp32) is the rate-limiting enzyme that breaks down heme to iron, carbon monoxide, and biliverdin, which is then metabolized to bilirubin by biliverdin reductase. In mammalians, heme oxygenase exists as two primary isoforms, the inducible isoform HO-1, and the constitutively expressed HO-2, both catalyzing the same reaction. HO-1 is expressed in erythrocyte and hemoglobin metabolizing tissues of the spleen, liver, and bone marrow, with localization to membranes of the ER, mitochondria, and caveolae2. HO-1 expression is induced in response to an array of oxidative stress-inducing factors, ncluding heat shock, heme accumulation, hypoxia, UV radiation, nitric oxide, cytokines, and heavy metals.
 
UniProt ID:P06762
 
GenBank ID:J02722
 

Product Literature References

Effects of inhaled nano-TiO2 aerosols showing two distinct agglomeration states on rat lungs: A. Noël, et al.; Toxicol. Lett. 214, 109 (2012), Application(s): ELISA using rat bronchoalveolar lavage fluid, Abstract;

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