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IMMUNOSET® HO-1 (mouse), ELISA development set

 
ADI-960-071 5x96 wells 510.00 USD
Do you need bulk/larger quantities?
 
  • Cost effective - coat your own plates and stretch your research budget
  • Quantitative - obtain fully quantitative statistically significant results
  • Sensitive - detect as little as 96 pg/mL HO-1 compared to microgram levels in Western blot
  • Reliable - obtain accurate results with thoroughly tested reagents in multiple complex sample matrices
The IMMUNOSET® HO-1 (mouse), EIA development set contains sufficient reagents for the development of 5 x 96 well EIA plates which provides analysis of ~200 samples in duplicate. Save time, money, and sample by developing your own EIA kits. Simply coat your plates and go! This kit provides superior accuracy with fully quantitative results compared to Western blot analysis and has low reactivity with related molecules.
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Product Specification

Alternative Name:Heme oxygenase 1
 
Sensitivity:96 pg/ml (range 0.195 - 12.5 ng/ml)
 
Assay Time:Plate Coating - Overnight + 1 hour; Assay - 3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of mouse HO-1 in cell lysates, plasma, serum, and tissue.
 
Species reactivity:Mouse
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Capture antibody, Standard, Detection antibody, SA-HRP
 
Scientific Background:Heme oxygenase (Hsp32) is the rate-limiting enzyme that breaks down heme to iron, carbon monoxide, and biliverdin, which is then metabolized to bilirubin by biliverdin reductase. In mammalians, heme oxygenase exists as two primary isoforms, the nducible isoform HO-1, and the constitutively expressed HO-2, both catalyzing the same reaction. HO-1 is expressed in erythrocyte and hemoglobin metabolizing tissues of the spleen, liver, and bone marrow, with localization to membranes of the ER, mitochondria, and caveolae2. HO-1 expression is induced in response to an array of oxidative stress-inducing factors, including heat shock, heme accumulation, hypoxia, UV radiation, nitric oxide, cytokines, and heavy metals.
 
UniProt ID:P14901
 

Product Literature References

Limonene and its ozone-initiated reaction products attenuate allergic lung inflammation in mice: J.S. Hansen, et al.; J. Immunotoxicol. 13, 1 (2016), Application(s): Lung tissue homogenization and determination of protein, HO-1 and cytokines, Abstract; Full Text
Nonhematopoietic Nrf2 dominantly impedes adult progression of sickle cell anemia in mice: S. Ghosh, et al.; JCL insight 1, e81090 (2016), Application(s): Measured concentration of HO-1 in the plasma and in lung homogenate, Abstract; Full Text
Astrocyte overexpression of heme oxygenase-1 improves outcome after intracerebral hemorrhage: J. Chen-Roetling, et al.; Stroke 46, 1093 (2015), Application(s): HO-1 levels in injected and contralateral striata, Abstract; Full Text
Dietary quercetin attenuates oxidant-induced endothelial dysfunction and atherosclerosis in apolipoprotein E knockout mice fed a high-fat diet: a critical role for heme oxygenase-1: Y. Shen, et al.; Free Radi. Biol. Med. 65, 908 (2013), Application(s): HO-1 levels in mouse aorta, Abstract;
Renal cortical lactate dehydrogenase: a useful, accurate, quantitative marker of in vivo tubular injury and acute renal failure: R.A. Zager, et al.; PLoS One 8, e66776 (2013), Application(s): ELISA using mouse plasma, Abstract; Full Text
Small molecule activators of the Nrf2-HO-1 antioxidant axis modulate heme metabolism and inflammation in BV2 microglia cells: R. Foresti, et al.; Pharmacol. Res. 76, 132 (2013), Application(s): HO-1 levels in BV2 cell lysates, Abstract;
Schizophrenia-like features in transgenic mice overexpressing human HO-1 in the astrocytic compartment: W. Song, et al.; J. Neurosci. 8, 32 (2012), Application(s): ELISA using transgenic mouse brain, Abstract; Full Text
Unregulated brain iron deposition in transgenic mice over-expressing HMOX1 in the astrocytic compartment: W. Song, et al.; J. Neurochem. 123, 325 (2012), Application(s): ELISA using transgenic mouse brain, Abstract;

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