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IHC diluent (secondary Ab)

 
ADI-950-245-0100 100 ml Inquire for pricing
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IHC diluent (secondary Ab) can be used to prepare secondary antibodies and negative controls. This diluent can be used with any secondary antibody irrespective of its source of origin. The IHC diluent (secondary Ab) is specifically designed to stabilize and dilute secondary antibodies used for immunohistochemistry and to reduce nonspecific background staining.

Product Specification

Formulation:Liquid. 10X secondary antibody diluent.
 
Appearance:Yellow solution.
 
Applications:IHC
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:In order to obtain optimal staining, correct dilution and selection of appropriate diluent is very critical. Ionic strength, pH of the environment,and the presence or absence of detergents can affect binding and the stability of the secondary antibody. The optimal pH together with stabilizing proteins in IHC diluent (secondary Ab) ensures maximum stability of secondary antibodies. The IHC diluent (secondary Ab) contains optimal concentrations of sodium chloride and detergents to prevent nonspecific binding and allow better penetration of secondary antibodies.

Interpretation of the results will be the sole responsibility of the user.
 
Protocol:Preparation of reagent: Dilute one part 10x IHC diluent (secondary Ab) with 9 parts deionized or distilled water.
Procedure:
1. Pour IHC diluent (secondary Ab) in a clean glass container.
2. Add concentrated secondary antibody to the IHC diluent (secondary Ab) and mix thoroughly.
3. Diluted secondary antibody conjugate is now ready to use and should be stored at 2-8°C for further use.
 

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