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IHC diluent (peroxidase II)

 
ADI-950-243-0100 100 ml Inquire for pricing
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IHC diluent (peroxidase II) is used to stabilize streptavidin-conjugated peroxidase at a higher dilution.

Product Specification

Formulation:Liquid. 10X peroxidase II diluent.
 
Appearance:Ruby red solution.
 
Applications:IHC
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Peroxidase is one of the most widely used enzyme markers in immunoassay systems. Because of the higher titer of labeled enzymes, diluted reagent has a tendency to start losing its activity after a few weeks even when stored at 2-8°C. IHC diluent (peroxidase II) is specially designed to solve this problem. When diluted in this diluent labeled peroxidase will not lose its activity. This diluent stabilizes the peroxidase conjugate and increases its shelf life for several years when stored at 2-8°C. IHC diluent (peroxidase II) contains immunologically inert protein to further stabilize the protein peroxidase conjugate and contains 0.1% Proclin 300 as a preservative.
 
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
 
Protocol:Preparation of reagent: Dilute one part 10x IHC diluent (peroxidase II) with 9 parts deionized or distilled water.
Procedure:
1. Pour diluted Peroxidase Diluent II in a clean glass container.
2. Add labeled Peroxidase conjugate to IHC diluent (peroxidase II) and mix thoroughly.
3. The diluted conjugate is now ready to use and should be stored at 2-8°C for further use.
 

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