Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

HIGHDEF® Red IHC Chromogen (HRP)

 
ADI-950-210-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 
The HIGHDEF® Red IHC Chromogen can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the immunodetection system reacts with hydrogen peroxide substrate to degrade it, which reacts with AEC, precipitating it at positive sites yielding a reddish brown colored product.

Product Specification

Formulation:Liquid. HIGHDEF® substrate (AEC, stable).
 
Appearance:Amber solution.
 
Applications:IHC
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Handling:Protect from light.
 
Technical Info/Product Notes:HIGHDEF® Red IHC Chromogen is a single, highly stable, AEC chromogen/substrate working solution. When used in conjunction with immunoperoxidase detection systems, AEC produces a red colored end product at positive sites, yielding strong contrast when combined with a blue hematoxylin counterstain. AEC has been well accepted among pathologists because it is less chemically hazardous than DAB. Specimens stained using HIGHDEF® Red IHC Chromogen cannot be dehydrated in ethanol and must be mounted in an aqueous-based mounting medium such as HIGHDEF® IHC mount (Prod. no. ADI-950-261).
 
Protocol:
  1. Once sections have been incubated with peroxidase, wash with wash buffer.
  2. Blot slides to remove excess buffer. Add enough drops of HIGHDEF® Red IHC Chromogen to cover tissue sections.
  3. Incubate for 5-15 minutes at room temperature. For best results, observe reaction under a microscope for signal development. Once desired signal-to-noise ratio is achieved, stop the reaction by washing slides in DI H2O.
 
ADI-950-210 IHC
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight CK antibody labeled with HIGHDEF® IHC substrate (AEC, stable) (Prod. no. ADI-950-210) produces a robust red color.
Please mouse over
ADI-950-210 IHC

Related Products

HIGHDEF® DAB Chromogen/Substrate Set 

Brown chromogen for use with HRP immunohistochemistry staining systems
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC105-0200 200 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® yellow IHC chromogen (HRP) 

Unique high definition yellow IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-170-0030 30 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® blue IHC chromogen (HRP) 

Unique high definition blue IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-151-0030 30 ml Inquire for pricing
 
ADI-950-151-0006 6 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® black IHC chromogen (HRP) 

Unique high definition black IHC chromogen for HRP-activated development.
IHC, ISH (in situ hybridization) | Print as PDF
 
ADI-950-171-0030 30 ml Inquire for pricing
 
ADI-950-171-0006 6 ml Inquire for pricing
Do you need bulk/larger quantities?
 

HIGHDEF® Hematoxylin 

Ready-to-use histologic stain with enhancers
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC106-0100 100 ml Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-rabbit) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC103-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

POLYVIEW® PLUS HRP (anti-mouse) reagent 

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC104-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

SAVIEW® PLUS HRP reagent 

High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications
IHC, ISH (in situ hybridization) | Print as PDF
 
ENZ-ACC102-0150 150 tests Inquire for pricing
Do you need bulk/larger quantities?
 

MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit 

Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample.
IHC | Print as PDF
 
ADI-950-100-0001 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Immunohistochemistry
Immunohistochemistry
Download as PDF

Brochures
Neuroscience Antibodies for IHC
Neuroscience Antibodies for IHC
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,