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IHC chromogen substrate (BCIP/NBT)

ADI-950-202-0015 15 ml Inquire for pricing
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IHC chromogen substrate (BCIP/NBT) can be used in conjunction with alkaline phosphatase based immunostaining systems. Alkaline phosphatase cleaves the phosphate group from BCIP to produce precipitating indoxyl group. This group then dimerizes resulting in the reduction of the NBT to produce a dark blue insoluble Formazan product.

Product Specification

Formulation:Liquid. BCIP/NBT solution.
Appearance:Amber solution.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Alkaline phosphatase, an enzyme derived from bovine intestinal mucosa, is often used as a label for in situ hybridization, immunohistochemistry, Southern and Northern hybridization and DNA sequencing. Detection of this enzyme requires the use of an insoluble colored substrate. We introduces a new stable, BCIP/NBT solution in ready to use form. It produces a blue colored end product at the positive sites which gives a good contrast with red nuclear fast counter stain. Specimens stained in BCIP/NBT can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium like HIGHDEF® IHC mount (Prod. no. ADI-950-261).
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user.
Protocol:1. Once tissue sections have been incubated with alkaline phosphatase, wash them with buffer thoroughly.
2. Blot the glass to remove excess of buffer and add enough drops of the IHC chromogen substrate (BCIP/NBT) to cover the tissue sections.
3. Incubate for 10-30 minutes at room temperature. For the best results, look under the microscope for the signal development. Once desired signal to noise ratio is achieved, stop the reaction by washing the slides in the wash buffer.

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