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IHC chromogen substrate (AEC)

ADI-950-200-0003 3 ml Inquire for pricing
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IHC chromogen substrate (AEC) can be used in conjunction with peroxidase based immunostaining systems. Peroxidase reacts with the 3% hydrogen peroxide substrate to degrade it, which in turn reacts with AEC to precipitate it at the positive sites giving red brown colored end product

Product Specification

Kit/Set Contains:AEC buffer, AEC chromogen, hydrogen peroxide substrate
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Technical Info/Product Notes:Even though DAB is much more sensitive chromogen compared to amino ethyl carbazol (AEC) because of its carcinogenic nature, some labs avoid using DAB. To resolve this problem, we have designed an AEC chromogen in liquid format. AEC is a chromogen of choice when performing immunoperoxidase staining. It has been well accepted amongst the pathologist because of its relatively less toxic nature compared to DAB. It produces a red colored end product at the positive sites which gives a good contrast with blue hematoxylin counter stain. Specimens stained in AEC can not be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium such as HIGHDEF® IHC mount (Prod. no. ADI-950-261).

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