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HIGHDEF® IHC enhancer (DAB)

 
ADI-950-181-0010 10 ml Inquire for pricing
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The HIGHDEF® IHC enhancer (DAB) is a DAB signal enhancer that can be used in conjunction with peroxidase based immunostaining systems.

Product Specification

Formulation:Liquid. 20X DAB chromogen enhancer.
 
Appearance:Clear solution.
 
Applications:IHC
 
Shipping:Ambient
 
Long Term Storage:Ambient
 
Scientific Background:DAB is a widely used chromogen for immunoperoxidase staining and immunoblotting. It has been well accepted among the pathologist because of its superior performance as compared to amino ethyl carbazol (AEC). DAB gives cleaner background as opposed to AEC. Specimens stained in DAB can be dehydrated in ethanol and cleared in xylene and can be mounted for permanent record keeping. Strength of signal generated during the immunostaining is the key to a good staining. HIGHDEF® IHC enhancer (DAB) is a stable liquid solution which increases the staining intensity several fold of tissue specimens stained with DAB and hence increases the efficiency of detection system.
 
Technical Info/Product Notes:Interpretation of the results will be the sole responsibility of the user. Used with IHC chromogen substrate (DAB) (Prod. No. ADI-950-203) and Peroxidase Block or as part of the MULTIVIEW® kits -- MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. No. ADI-950-100) or MULTIVIEW® (mouse-HRP/mouse-AP) IHC kit (Prod. No. ADI-950-101).
 
Protocol:
  1. Add one drop (50µl) of HIGHDEF® IHC enhancer (DAB) in 1ml of DAB incubation buffer.
  2. Add HIGHDEF® IHC substrate (DAB, high contrast) and Peroxidase Block (3% Hydrogen Peroxide) to the above buffer.
  3. Incubate tissue sections with the incubation medium.NOTE: Peroxidase Block is part of the MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. No. ADI-950-100) or MULTIVIEW® (mouse-HRP/mouse-AP) IHC kit (Prod. No. ADI-950-101) products.
 

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