HIGHDEF® blue IHC chromogen (AP) is intended to be used in conjunction with alkaline phosphatase (AP)-based immunostaining or in situ hybridization systems.
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil stained with a high molecular weight CK antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150) produces a distinct bright blue color.
Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) (Prod. no. ADI-950-211, brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150, blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) (Prod. no. ADI-950-141, red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, yellow), and methyl green nuclear counterstain.
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Product Details
| Applications: | IHC
ISH (in situ hybridization)
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| Contents: | HIGHDEF® blue IHC chromogen (AP) substrate buffer and chromogen, Empty mixing bottle |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Application Note
Three-color IHC Staining On Free-floating Sections From Human Brain |
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| Technical Info/Product Notes: | The HIGHDEF® blue IHC chromogen (AP) is a substrate-chromogen system designed to be used for either IHC or ISH when utilizing alkaline phosphatase. It has been formulated for increased stability of the working solution while producing a distinct bright blue color. It is insoluble in alcohol and xylene substitutes (both aliphatic hydrocarbon and citrus based); therefore sections can be dehydrated in alcohol, cleared in xylene substitute, and permanently mounted. However, we recommend air drying slides and then permanently mounting. This chromogen substrate system may be used for both automation and manual use. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Exploring human splenic red pulp vasculature in virtual reality: details of sheathed capillaries and the open capillary network: B.S. Steiniger, et al.; Histochem. Cell Biol.
155, 341 (2021),
Abstract;
Full Text
Innate immune modulation induced by EBV lytic infection promotes endothelial cell inflammation and vascular injury in scleroderma: A. Farina, et al.; Front. Immunol.
12, 651013 (2021),
Abstract;
Full Text
Phenotypic Changes in Macrophage Activation in a Model of Nonalcoholic Fatty Liver Disease using Microminipigs: D. Yoshii, et al.; J. Atheroscler. Thromb.
28, 844 (2021),
Abstract;
Plasma cells, plasmablasts, and AID+/CD30+ B lymphoblasts inside and outside germinal centres: details of the basal light zone and the outer zone in human palatine tonsils: B.S. Steiniger, et al.; Histochem. Cell Biol.
154, 55 (2020),
Abstract;
Full Text
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