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POLYVIEW® IHC reagent (mouse-AP)

High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF® chromogens.
 
ADI-950-110-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 
  • Biotin-free nanopolymer detection circumvents endogenous biotin background
  • High intensity color development delivers sharp, crisp staining
  • Customize with HIGHDEF® chromogens of your choice (sold separately)
The POLYVIEW® (mouse-AP) IHC detection reagent is a non-biotin one-step detection reagent suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The AP detection reagent may also be used with blood smears, cytosmears, and cell preparations.
ADI-950-110 IHC2
Immunohistochemistry analysis: Formalin fixed paraffin embedded human tonsil double stained sequentially with a CD20 mouse antibody, labeled with POLYVIEW® IHC reagent (mouse-AP) (Prod. no. ADI-950-110), seen with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150); then a CD3 mouse antibody labeled with POLYVIEW® IHC reagent (mouse-AP) (Prod. no. ADI-950-110), seen with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140) to produce a contrasting blue and red image.
ADI-950-110 IHC1
Immunohistochemistry analysis using MULTIVIEW® (mouse-HRP/mouse-AP) IHC kit (Prod. no. ADI-950-101): Formalin fixed paraffin embedded human tonsil double stained sequentially with a CK 5/14 mouse antibody, labeled with POLYVIEW® IHC reagent (mouse-HRP) (Prod. no. ADI-950-112), seen with HIGHDEF® IHC substrate (DAB, stable) (Prod. no. ADI-950-212); then a CD8 mouse antibody labeled with POLYVIEW® IHC reagent (mouse-AP) (Prod. no. ADI-950-110), seen with HIGHDEF® blue IHC chromogen (AP) (Prod. no. ADI-950-150) to produce a contrasting brown and blue image.
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ADI-950-110 IHC2 ADI-950-110 IHC1

Product Specification

Applications:IHC
 
Kit/Set Contains:Anti-Mouse AP detection reagent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:POLYVIEW® IHC detection reagent (mouse-AP) has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The POLYVIEW® IHC detection reagent (mouse-AP) enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. The kit is suitable for use with mouse all mouse antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.
 

Product Literature References

Expression of programmed cell death ligand 1 is associated with poor overall survival in patients with diffuse large B-cell lymphoma: J. Kiyasu, et al.; Blood 126, 2193 (2015), Application(s): IHC using FFPE tissue, Abstract; Full Text

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