- Biotin-free nanopolymer-based system for superior multiplex antigen detection
- Complete kit includes HIGHDEF® Chromogens for sensitive, crisp 2-color development
- Preserves precious tissue while reducing reagent and labor costs
- Expandable with additional chromogens (sold separately)
The MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit is a non-biotin one-step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and cryostat sections. The kit may also be used with blood smears, cytosmears, and cell preparations.
Immunohistochemical analysis: Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.
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Product Details
| Assay Time: | ~2 hours |
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| Applications: | IHC
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Peroxidase Block, POLYVIEW® IHC reagent (mouse-HRP), POLYVIEW® IHC reagent (rabbit-AP), HIGHDEF® IHC chromogen substrate (DAB, stable) buffer & chromogen, HIGHDEF® red IHC chromogen (AP) buffer & chromogen, Empty mixing bottles |
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| Technical Info/Product Notes: | The MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit has been developed by directly labeling anti-rabbit and anti-mouse immunoglobulins with enzymes using a proprietary tandem hyperlabelling technology. This ensures consistent and reproducible immunodetection of mouse and rabbit antibodies with a single reagent. Nuclear, cytoplasmic, and membrane antigens in different types of tissues can be readily detected. The MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates by being able to directly label mouse antibodies with HRP and rabbit antibodies with AP at the same time, all with significantly lower background staining. The kit is suitable for use with mouse and rabbit antibodies, both monoclonal and polyclonal. The reagents can be used for manual staining or with automated staining instruments and is specifically designed for multiplex immunohistochemical staining assays. |
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| Protocol: | MULTIVIEW® 4-color staining protocol available through our Technical Support webpages. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
AKAP12/Gravin is over-expressed in patients with ulcerative colitis: G. Fonseca-Camarillo, et al.; Immunol. Res.
69, 429 (2021),
Abstract;
Normalizing glucose levels reconfigures the mammary tumor immune and metabolic microenvironment and decreases metastatic seeding: H.A.M. Alsheikh, et al.; Cancer Lett.
517, 24 (2021),
Abstract;
Transglutaminase 2 promotes tumorigenicity of colon cancer cells by inactivation of the tumor suppressor p53: P. Malkomes, et al.; Oncogene
40, 4352 (2021),
Abstract;
Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization: T.H. Wang, et al.; Oncogenesis
9, 40 (2020),
Abstract;
Full Text
A Novel Three-Dimensional Immune Oncology Model for High-Throughput Testing of Tumoricidal Activity: H. Sherman, et al.; Front. Immunol.
9, 857 (2018),
Application(s): Lung carcinoma (A549/GFP) spheroids,
Abstract;
Full Text
Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease: J.K. Yamamoto-Furusho, et al.; J. Inflamm. (Lond.)
15, 13 (2018),
Abstract;
Full Text
Gelatinase B/Matrix Metalloproteinase-9 as Innate Immune Effector Molecule in Achalasia: J. Furuzawa-Carballeda, et al.; Clin. Transl. Gastroenterol.
9, 208 (2018),
Application(s): Tissue biopsies from achalasia patients,
Abstract;
Full Text
Nuclear factor-kappa B: Glucocorticoid-induced leucine zipper interface analogs suppress pathology in an Alzheimer's disease model: M. Srinivasan, et al.; Alzheimers Dement. (N. Y.)
4, 488 (2018),
Application(s): IHC on mouse brain sections,
Abstract;
Full Text
Prevalence of polyreactive innate clones among graft-infiltrating B cells in human cardiac allograft vasculopathy: D. Chatterjee, et al.; J. Heart Lung Transplant.
37, 385 (2018),
Application(s): Multiplex IHC staining on human cardiac tissue,
Abstract;
Challenges in enumeration of CTCs in breast cancer using techniques independent of cytokeratin expression: J. Castle, et al.; PLoS One
12, e0175547 (2017),
Application(s): Multiplex immunocytochemical(ICC) Oestrogen receptor alpha (Era)-based staining,
Abstract;
Full Text
Barrett's Oesophagus in an Achalasia Patient: Immunological Analysis and Comparison with a Group of Achalasia Patients: S. Torres-Landa, et al.; Case Rep. Gastrointest. Med.
2016, 5681590 (2016),
Application(s): Characterization of Tissue Regulatory Cell and CD4 Effector Cell Subsets by Immunohistochemistry,
Abstract;
Full Text
Gonadotropin-releasing hormone analogues inhibit leiomyoma extracellular matrix despite presence of gonadal hormones: M. Malik, et al.; Fertil. Steril.
105, 214 (2016),
Application(s): Multiplex IHC on 3D cultures,
Abstract;
Immunogenic FEAT protein circulates in the bloodstream of cancer patients: Y. Li, et al.; J. Transl. Med.
14, 275 (2016),
Application(s): Multiplex IHC staining on paraffin-embedded murine tumors,
Abstract;
Full Text
Mifepristone inhibits extracellular matrix formation in uterine leiomyoma: A. Patel, et al.; Fertil. Steril.
105, 1102 (2016),
Application(s): Multiplex IHC on 3D cultures,
Abstract;
Purinergic receptor X7 mediates leptin induced GLUT4 function in stellate cells in nonalcoholic steatohepatitis: V. Chandrashekaran, et al.; Biochim. Biophys. Acta
1862, 32 (2016),
Application(s): Multiplex IHC on mouse liver tissue sections,
Abstract;
Mitochondrial translocation of EGFR regulates mitochondria dynamics and promotes metastasis in NSCLC: T.F. Che, et al.; Oncotarget
6, 37349 (2015),
Application(s): Multiplex IHC on human lung cancer tissue sections,
Abstract;
Full Text
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