Alternative size available: ADI-930-001 (1x96 wells)
Ultrasensitive measurement, detecting as little as 8.26 pg/ml PGE2
Validated in a wide variety of sample matrices
Reliable and reproducible results lot-after-lot and day-after-day
Fully quantitative results that surpass semi-quantitative Western blot analysis
The PGE2 high sensitivity EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour.
Product Details
Alternative Name:
Prostaglandin E2
Sensitivity:
8.26 pg/ml (range 7.8 - 1,000 pg/ml)
Assay Time:
Overnight + 1 hour
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include plasma.
Wavelength:
405 nm
Species reactivity:
Species independent
Use/Stability:
Store all components at +4°C, except standard and conjugate at -20°C.
Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.
Leaching components and initial biocompatibility of novel bioactive restorative materials: S.N. Wuersching, et al.; Dent. Mater. 39, 293 (2023), Abstract;
A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers: S.N. Wuersching, et al.; Clin. Oral. Investig. 26, 6209 (2022), Abstract;
Design, synthesis and anti-inflammatory evaluation of aloe-emodin derivatives as potential modulators of Akt, NF-κB and JNK signaling pathways: H. Shang, et al.; Eur. J. Med. Chem. 238, 114511 (2022), Abstract;
Edwardsiella ictaluri T3SS Effector EseN Modulates Expression of Host Genes Involved in the Immune Response: L.P. Dubytska, et al.; Microorganisma 10, 1334 (2022), Abstract;
Effects of Different Cyclooxygenase Inhibitors on Prostaglandin E2 Production, Steroidogenesis and Ovulation of Bovine Preovulatory Follicles: A. Vernunft, et al.; J. Reprod. Dev. 68, 246 (2022), Abstract;
Human MuStem cells repress T-cell proliferation and cytotoxicity through both paracrine and contact-dependent pathways: M. Charrier, et al.; Stem Cells Res. Ther. 13, 7 (2022), Abstract;
Initial biocompatibility of novel resins for 3D printed fixed dental prostheses: S.N. Wuersching, et al.; Dent. Mater. J. 38, 1587 (2022), Abstract;
Anticancer Effects of Helminthostachys zeylanica Ethyl acetate Extracts on Human Gastric Cancer Cells through Downregulation of the TNF-α-activated COX-2-cPLA2-PGE2 Pathway: M.M. Tsai, et al.; J. Cancer 12, 7052 (2021), Abstract;
Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants 10, 2209 (2021), Abstract;
Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE2 pathway: S.G. Park, et al.; J. Vet. Sci. 22, e16 (2021), Application(s): ELISA on Feline adipose-tissue derived (fAT)-MSCs culture supernatant, Abstract; Full Text
Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling: I. Kowalczyk-Zięba, et al.; Animals (Basel) 11, 2636 (2021), Application(s): Rat Uterus tissue lysates, Abstract; Full Text
Mechanism of action and potential applications of selective inhibition of microsomal prostaglandin E synthase-1-mediated PGE2 biosynthesis by sonlicromanol’s metabolite KH176m: X. Jiang, et al.; Sci. Rep. 11, 880 (2021), Abstract;
N-acetylcysteine attenuates PGE 2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells: K. Nakamura, et al.; Dent. Mater. J. 40, 808 (2021), Abstract;
Synergistic effect of Korean red ginseng extract and GABA mixture on the IgE production in mice via Th1/Th2 cell balance: J.S. Lim, et al.; Food Sci. Biotechnol. 30, 1571 (2021), Abstract;
The inhibitory effect of AMP-activated protein kinase (AMPK) on chemokine and prostaglandin production in human endometrial stromal cells: Y. Kawano, et al.; Reprod. Biol. Endocrinol. 19, 188 (2021), Abstract;
The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention: A. Rapacz-Leonard, et al.; Sci. Rep. 10, 2089 (2020), Abstract; Full Text
Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019), Application(s): ELISA using culture supernatants, Abstract;
Group VIA phospholipase A2 deficiency in mice chronically fed with high-fat-diet attenuates hepatic steatosis by correcting a defect of phospholipid remodeling: A.C. Otto, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 662 (2019), Application(s): ELISA using liver homogenates and mouse serum, Abstract;
iPla2β deficiency in mice fed with MCD diet does not correct the defect of phospholipid remodeling but attenuates hepatocellular injury via an inhibition of lipid uptake: X. Zhu, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 677 (2019), Application(s): ELISA using liver homogenates, Abstract;
Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells: A. Roumane, et al.; BMC Cancer Biol. 19, 11 (2018), Abstract; Full Text
Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium: M.R. Rebordao, et al.; Theriogenology 113, 8 (2018), Abstract;
Human mesenchymal stromal cell sheets induce macrophages predominantly to an anti-inflammatory phenotype: P. Sukho, et al.; Stem Cells Dev. 27, 922 (2018), Abstract;
Impairment of the antifibrotic prostaglandin E2 pathway may influence neutrophil extracellular traps-induced fibrosis in the mare endometrium: M.R. Rebordão, et al.; Domest. Anim. Endocrinol. 67, 1 (2018), Application(s): PGE2 concentration in equine endometrial tissue culture, Abstract;
Monoacylglycerol lipase inhibitor JZL184 prevents HIV-1 gp120-induced synapse loss by altering endocannabinoid signaling: X. Zhang, et al.; Neuropharmacology 128, 269 (2018), Application(s): ELISA using culture supernatants, Abstract;
Pharmacological effects of N-[2-[[2-[2-[(2,6-dichlorophenyl)amino]phenyl]acetyl]oxy]ethyl]hyaluronamide (diclofenac Etalhyaluronate, SI-613), a novel sodium hyaluronate derivative chemically linked with diclofenac: K. Yoshioka, et al.; BMC Musculoskelet. Disord. 19, 157 (2018), Abstract; Full Text
The consequences of porcine IVM medium supplementation with follicular fluid become reflected in embryo quality, yield and gene expression patterns: P. Pawlak, et al.; Sci. Rep. 8, 15306 (2018), Abstract; Full Text
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants, Abstract;
Amelogenin induces M2 macrophage polarisation via PGE2/cAMP signalling pathway: K. Yamamichi, et al.; Arch. Oral Biol. 83, 241 (2017), Abstract;
Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease: S.F.H. de Witte, et al.; Stem Cell Res. Ther. 8, 140 (2017), Abstract; Full Text
Intramammary lipopolysaccharide infusion alters gene expression but does not induce lysis of the bovine corpus luteum: J. Lüttgenau, et al.; J. Dairy Sci. 99, 4018 (2016), Application(s): Bovine plasma, Abstract;
iPLA2β deficiency attenuates obesity and hepatic steatosis in ob/ob mice through hepatic fatty-acyl phospholipid remodeling: X. Deng, et al.; Biochim. Biophys. Acta 1861, 449 (2016), Abstract;
Prostaglandin E2 production during neonatal respiratory infection with mouse adenovirus type 1: M.C. Procario, et al.; Virus Res. 214, 26 (2016), Application(s): Measurement of PGE2 concentrations in lung homogenates, Abstract;
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells: I. Kowalczyk-Zieba, et al.; Prostaglandins Other Lipid Mediat. 121, 218 (2015), Application(s): PGE2 measurements, Abstract;
Anti-inflammatory effects of mapracorat, a novel selective glucocorticoid receptor agonist, is partially mediated by MAP kinase phosphatase-1 (MKP-1): T.R. Vollmer, et al.; J. Biol. Chem. 287, 35212 (2012), Application(s): PGE2 concentration measurements, Abstract;
Arzanol, a prenylated heterodimeric phloroglucinyl pyrone, inhibits eicosanoid biosynthesis and exhibits anti-inflammatory efficacy in vivo: J. Bauer, et al.; Biochem. Pharmacol. 81, 259 (2011), Application(s): ELISA using human cell culture supernatants, Abstract;
Elevated muscle interstitial levels of pain-inducing substances in symptomatic muscles in patients with polymyalgia rheumatica: F. Kreiner, et al.; Pain 152, 1127 (2011), Application(s): EIA using human plasma, Abstract;
Overexpression of aldo-keto reductase 1C2 is associated with disease progression in patients with prostatic cancer: K.H. Huang, et al.; Histopathology 57, 384 (2010), Application(s): Prostatic cancer tissue samples assayed, Abstract;
Identification of 5-lipoxygenase and microsomal prostaglandin E2 synthase-1 as functional targets of the anti-inflammatory and anti-carcinogenic garcinol: A. Koeberle, et al.; Biochem. Pharmacol. 77, 1513 (2009), Application(s): EIA using human plasma, Abstract;
Expression of the Embryonic Lethal Abnormal Vision-like Protein HuR in Human Mesothelioma Association With Cyclooxygenase-2 and Prognosis: A. Baldi, et al. ; Cancer 113, 2761 (2008), Application(s): EIA using human culture supernatants, Abstract;
Predictive population pharmacokinetic/pharmacodynamic model for anovel COX-2 inhibitor: S. Rohatagi, et al. ; J. Clin. Pharmacol. 47, 358 (2007), Application(s): EIA using human plasma, Abstract;
Spinal inflammatory hyperalgesia is mediated by prostaglandin E receptors of the EP2 subtype: H. Reinold, et al.; J. Clin. Invest. 115, 673 (2005), Application(s): PGE2 from the thoracolumbar segment of the spinal cord was measured, mice, Abstract; Full Text
Effect of a Selective Cyclooxygenase-2 Inhibitor, Nimesulide, on the Growth of Lung Tumors and Their Expression of Cyclooxygenase-2 and Peroxisome Proliferator- Activated Receptor-{gamma}: M.S. Shaik, et al. ; Clin. Cancer Res. 10, 1521 (2004), Application(s): EIA using mouse tissue, Abstract;
Differential Expression of Platelet-Activating Factor Acetylhydrolase in Macrophages and Monocyte-Derived Dendritic Cells: S. Al-Darmaki, et al. ; J. Immunol. 170, 167 (2003), Application(s): EIA using human culture supernatants, Abstract;
Inhibition of Cyclooxygenase-2 by Rofecoxib Attenuates the Growth and Metastatic Potential of Colorectal Carcinoma in Mice: M. Yao, et al.; Cancer Res. 63, 586 (2003), Application(s): Concentration of PGE2 in splenic tumor cystosolic fractions were determined using mouse tissue, Abstract;
Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al.; J. Immunol. 170, 4738 (2003), Application(s): PGE2 production was determined by enzyme immunoassay analysis using rat tissue, Abstract;
Iloprost suppresses connective tissue growth factor production in fibroblasts and in the skin of scleroderma patients: R. Stratton, et al. ; J. Clin. Invest. 108, 241 (2001), Application(s): EIA using human culture supernatants, Abstract;
Promoter Methylation Regulates Helicobacter pylori-stimulated Cyclooxygenase-2 Expression in Gastric Epithelial Cells: M. Akhtar, et al.; Cancer Res. 61, 2399 (2001), Application(s): EIA using cell culture supernatant, Abstract; Full Text
Inflammatory Agonists Induce Cyclooxygenase Type 2 Expression by Human Neutrophils: C.G. Maloney, et al.; J. Immunol. 160, 1402 (1998), Application(s): EIA using human cell culture supernatant, Abstract;
