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PGE2 high sensitivity ELISA kit

Ultrasensitive PGE2 ELISA kit for inflammation and eicosanoid research.
 
ADI-931-001 5x96 wells 1,512.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-930-001 (1x96 wells)
  • Ultrasensitive measurement, detecting as little as 8.26 pg/ml PGE2
  • Validated in a wide variety of sample matrices
  • Reliable and reproducible results lot-after-lot and day-after-day
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The PGE2 high sensitivity EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour.

Product Specification

Alternative Name:Prostaglandin E2
 
Sensitivity:8.26 pg/ml (range 7.8 - 1,000 pg/ml)
 
Assay Time:Overnight + 1 hour
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include plasma.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.
 

Product Literature References

Amelogenin induces M2 macrophage polarisation via PGE2/cAMP signalling pathway: K. Yamamichi, et al.; Arch. Oral Biol. 83, 241 (2017), Abstract;
Prostaglandin E2 production during neonatal respiratory infection with mouse adenovirus type 1: M.C. Procario, et al.; Virus Res. 214, 26 (2016), Application(s): Measurement of PGE2 concentrations in lung homogenates, Abstract;
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells: I. Kowalczyk-Zieba, et al.; Prostaglandins Other Lipid Mediat. 121, 218 (2015), Application(s): PGE2 measurements, Abstract;
Anti-inflammatory effects of mapracorat, a novel selective glucocorticoid receptor agonist, is partially mediated by MAP kinase phosphatase-1 (MKP-1): T.R. Vollmer, et al.; J. Biol. Chem. 287, 35212 (2012), Application(s): PGE2 concentration measurements, Abstract;
Arzanol, a prenylated heterodimeric phloroglucinyl pyrone, inhibits eicosanoid biosynthesis and exhibits anti-inflammatory efficacy in vivo: J. Bauer, et al.; Biochem. Pharmacol. 81, 259 (2011), Application(s): ELISA using human cell culture supernatants, Abstract;
Elevated muscle interstitial levels of pain-inducing substances in symptomatic muscles in patients with polymyalgia rheumatica: F. Kreiner, et al.; Pain 152, 1127 (2011), Application(s): EIA using human plasma, Abstract;
Overexpression of aldo-keto reductase 1C2 is associated with disease progression in patients with prostatic cancer: K.H. Huang, et al.; Histopathology 57, 384 (2010), Application(s): Prostatic cancer tissue samples assayed, Abstract;
Identification of 5-lipoxygenase and microsomal prostaglandin E2 synthase-1 as functional targets of the anti-inflammatory and anti-carcinogenic garcinol: A. Koeberle, et al.; Biochem. Pharmacol. 77, 1513 (2009), Application(s): EIA using human plasma, Abstract;
Expression of the Embryonic Lethal Abnormal Vision-like Protein HuR in Human Mesothelioma Association With Cyclooxygenase-2 and Prognosis: A. Baldi, et al. ; Cancer 113, 2761 (2008), Application(s): EIA using human culture supernatants, Abstract;
Predictive population pharmacokinetic/pharmacodynamic model for anovel COX-2 inhibitor: S. Rohatagi, et al. ; J. Clin. Pharmacol. 47, 358 (2007), Application(s): EIA using human plasma, Abstract;
Spinal inflammatory hyperalgesia is mediated by prostaglandin E receptors of the EP2 subtype: H. Reinold, et al.; J. Clin. Invest. 115, 673 (2005), Application(s): PGE2 from the thoracolumbar segment of the spinal cord was measured, mice, Abstract; Full Text
Effect of a Selective Cyclooxygenase-2 Inhibitor, Nimesulide, on the Growth of Lung Tumors and Their Expression of Cyclooxygenase-2 and Peroxisome Proliferator- Activated Receptor-{gamma}: M.S. Shaik, et al. ; Clin. Cancer Res. 10, 1521 (2004), Application(s): EIA using mouse tissue, Abstract;
Differential Expression of Platelet-Activating Factor Acetylhydrolase in Macrophages and Monocyte-Derived Dendritic Cells: S. Al-Darmaki, et al. ; J. Immunol. 170, 167 (2003), Application(s): EIA using human culture supernatants, Abstract;
Inhibition of Cyclooxygenase-2 by Rofecoxib Attenuates the Growth and Metastatic Potential of Colorectal Carcinoma in Mice: M. Yao, et al.; Cancer Res. 63, 586 (2003), Application(s): Concentration of PGE2 in splenic tumor cystosolic fractions were determined using mouse tissue, Abstract;
Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al.; J. Immunol. 170, 4738 (2003), Application(s): PGE2 production was determined by enzyme immunoassay analysis using rat tissue, Abstract;
Iloprost suppresses connective tissue growth factor production in fibroblasts and in the skin of scleroderma patients: R. Stratton, et al. ; J. Clin. Invest. 108, 241 (2001), Application(s): EIA using human culture supernatants, Abstract;
Promoter Methylation Regulates Helicobacter pylori-stimulated Cyclooxygenase-2 Expression in Gastric Epithelial Cells: M. Akhtar, et al.; Cancer Res. 61, 2399 (2001), Application(s): EIA using cell culture supernatant, Abstract; Full Text
Inflammatory Agonists Induce Cyclooxygenase Type 2 Expression by Human Neutrophils: C.G. Maloney, et al.; J. Immunol. 160, 1402 (1998), Application(s): EIA using human cell culture supernatant, Abstract;

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