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PGF high sensitivity ELISA kit

Most sensitive PGF ELISA kit available for prostaglandin research.
 
ADI-930-069 96 wells 366.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-931-069 (5x96 wells)
  • Convenient - assay most samples without extraction
  • Robust - fully validated in multiple complex sample matrices
  • Results from up to 37 samples in duplicate per 96-well plate
The PGF high sensitivity EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 3 hours. Absorbance is read at 405 nm. Simply the most sensitive quantification fo PGF commercially available in an enzyme immunoassay. Assay more samples without extraction.
PGF2α high sensitivity ELISA kit Kit graph
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PGF2α high sensitivity ELISA kit Kit graph

Product Specification

Alternative Name:Prostaglandin F2α
 
Sensitivity:0.98 pg/ml (range 1.95 - 2,000 pg/ml)
 
Assay Time:Overnight + 3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of PGF in culture supernatants, milk, plasma, serum, saliva, and urine from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.
 

Product Literature References

Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019), Application(s): ELISA using culture supernatants, Abstract;
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic luteal cells: I. Kowalczyk-Zieba, et al.; Prostaglandins Other Lipid Mediat. 121, 218 (2015), Application(s): PGF2α measurements, Abstract;

Related Products

PGF high sensitivity ELISA kit 

Most sensitive PGF ELISA kit available for prostaglandin research.
ELISA, Colorimetric detection | Print as PDF
 
ADI-931-069 5x96 wells 1,480.00 USD
Do you need bulk/larger quantities?
 

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