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Homogeneous mix and read assay
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Measures nitrate and nitrite separately
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Fast results in 10 to 40 minutes from up to 39 samples in duplicate
The Nitric Oxide (NO2-/NO3-), detection kit is a complete kit for the quantitative determination of nitrate and nitrite concentrations. Absorbance is read at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.
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Product Details
| Sensitivity: | (Nitrite) 0.222 µmole/L; (Nitrate) 0.625 µmole/L (range (Nitrite) 3.125 - 200 µmole/L; (Nitrate) 3.125 - 100 µmole/L) |
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| Applications: | Colorimetric detection
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| Application Notes: | For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species. |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at 4°C, except nitrate reductase at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Microtiter plate, Nitrate Reductase, Nitrate Reductase Storage Buffer, NADH, Nitrate Standard, Nitrite Standard, Reaction buffer concentrate, Griess Reagent I, Griess Reagent II |
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| Scientific Background: | Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Activation of TRPV1 improves natriuresis and salt sensitivity in high-fat diet fed mice: B. Zhong, et al.; Biochem. Pharmacol.
203, 115190 (2022),
Abstract;
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat.
155, 106565 (2021),
Abstract;
Independent and inter-dependent immunoregulatory effects of NCF1 and NOS2 in experimental autoimmine encephalomyelitis: J. Zhong, et al.; J. Neuroinflammation
17, 113 (2020),
Abstract;
Full Text
Influence of acute high-intensity exercise on salivary nitric oxide levels: R. Sone, et al.; J. Oral Sci.
61, 307 (2019),
Abstract;
Full Text
Didymin ameliorates hepatic injury through inhibition of MAPK and NF-κB pathways by up-regulating RKIP expression: Q. Huang, et al.; Int. Immunopharmacol.
42, 130 (2017),
Abstract;
Studies on the Anti-Oxidative Function of trans-Cinnamaldehyde-Included β-Cyclodextrin Complex: M. Davaatseren, et al.; Molecules
22, E1868 (2017),
Abstract;
Full Text
Dimethyl sulfoxide attenuates nitric oxide generation via modulation of cationic amino acid transporter-1 in human umbilical vein endothelial cells: O.S. Bentur; Cryobiology
16, 30086 (2016),
Application(s): Nitrite/nitrate determination,
Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum nitric oxide levels,
Molecular Mechanism of Silver Nanoparticles-Induced Human Osteoblast Cell Death: Protective Effect of Inducible Nitric Oxide Synthase Inhibitor: E. Zielinska, et al.; PLoS One
11, e0164137 (2016),
Application(s): Measuring levels of NO in the presence or absence of AgNPs,
Abstract;
Full Text
Post-ischemic conditioning does not reduce muscle injury after tourniquet induced ischemia-reperfusion injury in rats: V.J. Mase Jr., et al.; Am. J. Emerg. Med
34, 2065 (2016),
Application(s): Total nitric oxide concentrations were measured in rat tissue samples,
Abstract;
Quinapril decreases antifibrinolytic and prooxidative potential of propofol in arterial thrombosis in hypertensive rats: M. Wojewodzka-Zeleziankowicz, et al.; J. Renin Angiotensin Aldosterone Syst.
17, 1470320316647239 (2016),
Application(s): Measured rat plasma NO levels,
Abstract;
Full Text
Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs: E. Petitdidier, et al.; PLoS Negl. Trop. Dis.
10, e0004614 (2016),
Application(s): Cytokine and nitric oxide measurements,
Abstract;
Full Text
Tangeretin Alleviates Cisplatin-Induced Acute Hepatic Injury in Rats: Targeting MAPKs and Apoptosis: H. A. Omar, et al.; PLoS One
11, e0151649 (2016),
Application(s): Nitric oxide levels were measured in liver homogenates,
Abstract;
Full Text
Ginger and Marshmallow Extracts Can Protect against Pyloric Ligation-Induced Peptic Ulcer in Rats: S. S. Zaghlool, et al.; Int. J. Pharm. Sci. Drug Res.
7, 143 (2015),
Application(s): Assay using rat gastric mucosal homogenate,
Full Text
Investigation of the Plasma Nitrite Levels and Oxidant–Antioxidant Status in Obstructive Sleep Apnea Syndrome: H. Bozkurt, et al.; Noro Psikiyatr. Ars.
52, 221 (2015),
Application(s): Assay using human plasma,
Abstract;
Full Text
Effects of systemic chlorogenic acid on random-pattern dorsal skin flap survival in diabetic rats: D. Bagdas, et al.; Biol. Pharm. Bull.
37, 361 (2014),
Abstract;
Angiostatin production increases in response to decreased nitric oxide in aging rat kidney: M. Satoh, et al.; Lab. Invest.
93, 334 (2013),
Abstract;
