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Nitric Oxide (NO2-/NO3-) detection kit

 
ADI-917-010 2x96 wells 243.00 USD
Do you need bulk/larger quantities?
 
  • Homogeneous mix and read assay
  • Measures nitrate and nitrite separately
  • Fast results in 10 to 40 minutes from up to 39 samples in duplicate
The Nitric Oxide (NO2-/NO3-), detection kit is a complete kit for the quantitative determination of nitrate and nitrite concentrations. Absorbance is read at 540-570 nm. Only 0.050 ml diluted sample needed per well saves precious sample.
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Product Specification

Sensitivity:(Nitrite) 0.222 µmole/L; (Nitrate) 0.625 µmole/L (range (Nitrite) 3.125 - 200 µmole/L; (Nitrate) 3.125 - 100 µmole/L)
 
Applications:Colorimetric detection
 
Application Notes:For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at +4°, except nitrate reductase at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Nitrate Reductase, Nitrate Reductase Storage Buffer, NADH, Nitrate Standard, Nitrite Standard, Reaction buffer concentrate, Griess Reagent I, Griess Reagent II
 
Scientific Background:Nitric oxide (NO) is a major mammalian secretory product that initiates host defense, homeostatic and developmental functions by either direct effect or intercellular signaling. NO is the product of a five-electron oxidation of the amino acid L-arginine mediated by nitric oxide synthase. As a direct effector, NO is thought to activate regulatory proteins, kinases and proteases that are directed by reactive oxygen intermediates. Activation of the immune system is associated with an increase in macrophage NO production. Soluble NO exerts a variety of homeostatic influences as an activator of soluble guanylyl cyclase, a neuronal potentiator, a peripheral nervous system neurotransmitter, and a contraction regulator of both smooth muscle and vascular tissue. In addition, NO has been linked to the formation of olfactory and synaptic memories and remodeling.
 

Product Literature References

Didymin ameliorates hepatic injury through inhibition of MAPK and NF-κB pathways by up-regulating RKIP expression: Q. Huang, et al.; Int. Immunopharmacol. 42, 130 (2017), Abstract;
Dimethyl sulfoxide attenuates nitric oxide generation via modulation of cationic amino acid transporter-1 in human umbilical vein endothelial cells: O.S. Bentur; Cryobiology 16, 30086 (2016), Application(s): Nitrite/nitrate determination, Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. (2016), Application(s): Measured rat serum nitric oxide levels,
Molecular Mechanism of Silver Nanoparticles-Induced Human Osteoblast Cell Death: Protective Effect of Inducible Nitric Oxide Synthase Inhibitor: E. Zielinska, et al.; PLoS One 11, e0164137 (2016), Application(s): Measuring levels of NO in the presence or absence of AgNPs, Abstract; Full Text
Post-ischemic conditioning does not reduce muscle injury after tourniquet induced ischemia-reperfusion injury in rats: V.J. Mase Jr., et al.; Am. J. Emerg. Med 34, 2065 (2016), Application(s): Total nitric oxide concentrations were measured in rat tissue samples, Abstract;
Quinapril decreases antifibrinolytic and prooxidative potential of propofol in arterial thrombosis in hypertensive rats: M. Wojewodzka-Zeleziankowicz, et al.; J. Renin Angiotensin Aldosterone Syst. 17, 1470320316647239 (2016), Application(s): Measured rat plasma NO levels, Abstract; Full Text
Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs: E. Petitdidier, et al.; PLoS Negl. Trop. Dis. 10, e0004614 (2016), Application(s): Cytokine and nitric oxide measurements, Abstract; Full Text
Tangeretin Alleviates Cisplatin-Induced Acute Hepatic Injury in Rats: Targeting MAPKs and Apoptosis: H. A. Omar, et al.; PLoS One 11, e0151649 (2016), Application(s): Nitric oxide levels were measured in liver homogenates, Abstract; Full Text
Ginger and Marshmallow Extracts Can Protect against Pyloric Ligation-Induced Peptic Ulcer in Rats: S. S. Zaghlool, et al.; Int. J. Pharm. Sci. Drug Res. 7, 143 (2015), Application(s): Assay using rat gastric mucosal homogenate, Full Text
Investigation of the Plasma Nitrite Levels and Oxidant–Antioxidant Status in Obstructive Sleep Apnea Syndrome: H. Bozkurt, et al.; Arch. Neuropsychiatr. (2015), Application(s): Assay using human plasma, Full Text

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