Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Diglycyl Lysine monoclonal antibody (GX41)

Highly specific monoclonal for ubiquitin remnant profiling via immunoprecipitation.
 
ADI-908-310-0100 100 µg 235.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Clone:GX41
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Diglycine-modified histone from calf thymus, Type III-S, lysine rich fraction.
 
Species reactivity:Species independent
 
Specificity:Recognizes diglycyl modified lysine, a ubiquitin remnant detectable after trypsin digestion.
 
Applications:IP, WB
 
Purity Detail:Protein G purified.
 
Formulation:Liquid. In PBS containing 0.1% sodium azide.
 
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Protein ubiquitination is a fundamentally important post-translational modification that regulates myriad cell signaling pathways in eukaryotes. Alterations in protein ubiquitination have been linked to cancers and neurodegenerative, cardiovascular, immunological, and inflammatory diseases.

Identification of exact ubiquitinylation sites is challenging under physiological conditions, but the recent development of an immunoprecipitation approach allows for enrichment and identification of peptides derived from the ubiquitinated portion of proteins.

When ubiquitinated protein is digested with trypsin a diglycine residue remains at any lysine site of ubiquitin attachment. Diglycyl Lysine, mAb (GX41) facilitates easy capture and enrichment of peptides derived from ubiquitinated proteins by recognizing the diglycyl-lysine residue with no detectable reactivity to non modified lysines.
 

Product Literature References

Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues: S.A. Wagner, et al.; Mol. Cell. Proteomics 11, 1578 (2012), Abstract; Full Text
Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass: L.K. Povlsen, et al.; Nat. Cell Biol. 14, 1089 (2012), Abstract;
A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles: S.A. Wagner, et al.; Mol. Cell. Proteomics 10, M111.013284 (2011), Abstract; Full Text
The new landscape of protein ubiquitination: G. Xu, et al.; Nat. Biotechnol. 290, 1098 (2011), Abstract;
Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling: G. Xu, et al.; Nat. Biotechnol. 28, 868 (2010), Abstract; Full Text

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

Brochures
Epigenetics
Epigenetics
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,