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Irisin (human), (recombinant) (active)

First to market active Irisin.
 
ADI-908-307-0010 10 µg 357.00 USD
 
ADI-908-307-3010 3x10 µg 670.00 USD
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  • First to market active Irisin
  • Multifunctional protein validated for activity assay, ELISA, SDS-PAGE and Western blot
  • Important regulatory protein for metabolism and neurogenesis

Product Specification

Alternative Name:FNDC5, FRCP2
 
MW:~25kDa
 
Source:Produced in CHO cells. aa 32-143, also known as Irisin, comprises the majority of the extracellular domain of FNDC5. It is fused at the N-terminus to a FLAG®-tag with an 8 aa linker between the FLAG®-tag and Irisin.
 
UniProt ID:Q8NAU1
 
Formulation:Liquid. In 1X PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.01EU/µg
 
Biological Activity:Treatment of 3T3L1 with 2µg/ml Irisin induces UCP-1 expression.
 
Applications:ELISA, WB
Activity assay, SDS-PAGE
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Use/Stability:Stable for up to a week when stored at +4°C and at least 6 months when stored at -80°C.
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:Irisin is a hormone that is involved in converting white adipose tissue into brown adipose tissue after physical exercise. It is formed after the membrane bound protein fibronectin type III domain containing protein 5 (FNDC5) is cleaved resulting in a secreted protein. FNDC5 is found in muscle tissue and is activated during physical exertion via the protein PGC1a. Soluble Irisin has also been shown to up regulate UCP-1 gene expression in mice. UCP-1 is a mitochondrial transporter protein and a marker of brown adipose tissue conversion. This suggests that Irisin plays an important role in energy regulation and furthermore may lead to improvements in glucose regulation, insulin sensitivity, hypertension, and obesity.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 
ADI-908-307 Fig1
Figure 1: Coomassie stained SDS-PAGE analysis of Irisin (human) (rec). Lane 1: Molecular weight marker, Lane 2: 1µg Irisin.
ADI-908-307 Fig2
Figure 2: Western blot analysis of Irisin (human) (rec) against mouse anti-flag antibody. Lane 1: Molecular weight marker, Lane 2: 0.1µg Irisin.
ADI-908-307 Fig3
Figure 3: Western blot analysis of Irisin (human) (rec) against rabbit anti-FNDC5 antibody. Lane 1: Molecular weight marker, Lane 2: 0.1µg Irisin.
ADI-908-307 Fig4
Figure 4: Stimulation of UCP-1 after treatment with Irisin (human) (rec). 3T3L1 cells were treated with or without 2µg/ml Irisin. After 48 hours the cells were lysed, normalized to total protein, and run on an SDS-PAGE. Gels were transferred to nitrocellulose and blotted against rabbit anti-UCP-1 antibody.
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ADI-908-307 Fig1 ADI-908-307 Fig2 ADI-908-307 Fig3 ADI-908-307 Fig4

Product Literature References

Irisin inhibition of growth hormone secretion in cultured tilapia pituitary cells: A. Lian, et al.; Mol. Cell Endocrinol. 439, 395 (2017), Application(s): Molecular characteristics, tissue expression, and biological actions of irisin on GH secretion in talipia, Abstract;

General Literature References

A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis.: Bostrom P, Wu J, Jedrychowski MP, Korde A, Ye L, et al.; Nature 481, 463–468 (2012),

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