Product Specification
| Alternative Name: | MT-SP1, Membrane-type serine protease 1, ST14, Suppression of tumorigenicity protein 14, TADG-15, Tumor-associated differentially expressed gene 15 protein, SNC19 |
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| MW: | ~30kDa |
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| Source: | Produced in insect cells. Human recombinant matriptase (aa 619-854) is fused to a His-tag. Produced in a baculovirus expression system. |
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| EC: | 3.4.21.109 |
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| UniProt ID: | Q9Y5Y6 |
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| GenBank ID: | 6647302 |
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| Gene/Protein Identifier: | 6768 (Gene ID) |
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| Formulation: | Liquid. In 50mM TRIS containing 10% glycerol. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Activity: | Not active. Inhibited with AEBSF for stability. |
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| Applications: | WB
in vitro Assay
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| Application Notes: | Western blot control. |
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| Solubility: | Soluble in 1X PBS or water |
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| Shipping: | Shipped on Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Scientific Background: | The protein encoded by the Matriptase gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion and metastasis. |
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SDS-PAGE Analysis. Lane 1: Marker, Lane 2: Matriptase His-tag recombinant protein. Matriptase His-tag recombinant protein was purified over a Ni-NTA bead column, washed, and then eluted into 0.5ml fractions which were then combined for the final purified product.
Western blot analysis of Matriptase (human), (recombinant) (His-tag) (Prod. No. ADI-908-301) probed with Matriptase, mAb (D11) (Prod. No. ADI-905-1008) (lane 2) or Matriptase, mAb (B12) (Prod. No. ADI-905-1007) (lane 3). Lane 1 includes a representative MW Marker.
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For Research Use Only. Not for use in diagnostic procedures.
