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Matriptase (human), (recombinant) (His-tag)

 
ADI-908-301-0010 10 µg 310.00 USD
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Product Specification

Alternative Name:MT-SP1, Membrane-type serine protease 1, ST14, Suppression of tumorigenicity protein 14, TADG-15, Tumor-associated differentially expressed gene 15 protein, SNC19
 
MW:~30kDa
 
Source:Produced in insect cells. Human recombinant matriptase (aa 619-854) is fused to a His-tag. Produced in a baculovirus expression system.
 
EC:3.4.21.109
 
UniProt ID:Q9Y5Y6
 
GenBank ID:6647302
 
Gene/Protein Identifier:6768 (Gene ID)
 
Formulation:Liquid. In 50mM TRIS containing 10% glycerol.
 
Purity:≥90% (SDS-PAGE)
 
Applications:WB
in vitro Assay
 
Application Notes:Western blot control.
 
Solubility:Soluble in 1X PBS or water
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The protein encoded by the Matriptase gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion and metastasis.
 
ADI-908-301 SDS-PAGE
SDS-PAGE Analysis. Lane 1: Marker, Lane 2: Matriptase His-tag recombinant protein. Matriptase His-tag recombinant protein was purified over a Ni-NTA bead column, washed, and then eluted into 0.5ml fractions which were then combined for the final purified product.
ADI-908-301 WB
Western blot analysis of Matriptase (human), (recombinant) (His-tag) (Prod. No. ADI-908-301) probed with Matriptase, mAb (D11) (Prod. No. ADI-905-1008) (lane 2) or Matriptase, mAb (B12) (Prod. No. ADI-905-1007) (lane 3). Lane 1 includes a representative MW Marker.
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ADI-908-301 SDS-PAGE ADI-908-301 WB

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