SDS-PAGE Analysis. Lane 1: Marker, Lane 2: KLK8 Fraction 4 from column (22µl), Lane 3: KLK8 Fraction 5 from column (22µl), Lane 4: KLK8 Fraction 6 from column (22µl), Lane 5: KLK8 Fraction 7 from column (22µl), Lane 6: KLK8 Fraction 9 from column (22µl). KLK8 His-tag recombinant protein was purified over a Ni-NTA bead column, washed, and then eluted into 0.5ml fractions which were then combined for the final purified product.
Western blot analysis of KLK8 (human), (recombinant) (His-tag) (Prod. No. ADI-908-300) probed with KLK8, mAb (BE) (Prod. no. ADI-905-1000) (lane 2) or KLK8, mAb (B12) (Prod. no. ADI-905-1005) (lane 3). Lane 1 includes a representative MW Marker.