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Creatinine colorimetric detection kit

Low price, rapid creatinine detection kit for diabetes, cardiovascular and renal research.
 
ADI-907-030A 2x96 wells 213.00 USD
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Replaces Prod. #: ADI-907-030

  • Easy-to-use one-step creatinine detection
  • Fast homogeneous mix-and-ready assay with no wash steps, results in just 30 minutes
  • Accurate and calibrated to NIST creatinine standard
The Creatinine colorimetric detection kit utilizes a single-step liquid detection reagent that is safer and less time consuming than other assay methods. This kit is calibrated against the NIST standard and offers reproducible results with less than 6% inter- and intra-assay variation.
907-030A diagram
907-030A std curve
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907-030A diagram 907-030A std curve

Product Specification

Sensitivity:0.042 mg/dl (range 0.31 - 20 mg/dl)
 
Assay Time:30 minutes
 
Applications:Colorimetric detection
 
Application Notes:For the quantitative determination of urinary creatinine from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Standard, Detection Reagent
 
Scientific Background:Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.
 

Product Literature References

Moderate intake of docosahexaenoic acid raises plasma and platelet vitamin E levels in cystic fibrosis patients: E. Vericel, et al.; Prostaglandins Leukot. Essent. Fatty Acids 115, 41 (2016), Application(s): Urine, Abstract; Full Text
Liver but not adipose tissue is responsive to the pattern of enteral feeding: Y.F. Otero, et al.; Physiol. Rep. 2, e00250 (2014), Application(s): Assay using mouse urine, Abstract; Full Text
Mice Do Not Habituate to Metabolism Cage Housing–A Three Week Study of Male BALB/c Mice: O. Kalliokoski, et al.; PLoS One 8, e58460 (2013), Application(s): Assay using mouse urine, Abstract; Full Text
Potential trends in Attention Deficit Hyperactivity Disorder (ADHD) drug use on a college campus: wastewater analysis of amphetamine and ritalinic acid: D.A. Burgard, et al.; Sci. Total Environ. 450, 242 (2013), Application(s): Assay using acidified wastewater, Abstract;

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