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Catalase fluorometric detection kit

 
ADI-907-027 500 tests 350.00 USD
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  • Homogeneous - mix-and-read format easily adaptable to HTS applications
  • Sensitive - fluorescent detection system
  • Fast - results in just 1 hour
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.
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Product Specification

Sensitivity:(range 0- 4 U/ml)
 
Applications:Fluorescent detection, HTS
 
Application Notes:For the measurement of catalase in cell lysates from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Reaction buffer concentrate, Detection Reagent, Horseradish peroxidase concentrate, Hydrogen peroxide, Catalase enzyme
 
Scientific Background:Catalase is an antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system and functions to alleviate oxidative damage by catalyzing metabolism of the peroxide substrate. Accumulation of H2O2 can result in cellular damage through oxidation of proteins, DNA, and lipids, and has been linked to numerous pathologies including inflammation, cancer, diabetes, Parkinson’s disease, and Alzheimer’s.
 
UniProt ID:P00432
 

Product Literature References

Exploration of scalp surface lipids reveals squalene peroxide as a potential actor in dandruff condition: R. Jourdain, et al.; Arch. Dermatol. Res. 308, 153 (2016), Application(s): Determined catalase activity, Abstract;
Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells: S.J. Choi, et al.; Int. J. Nanomedicine 10, 3217 (2015), Application(s): Assay using human lung alveolar carcinoma epithelial cells, Abstract; Full Text
Cystine dimethylester loading promotes oxidative stress and a reduction in ATP independent of lysosomal cystine accumulation in a human proximal tubular epithelial cell line: R. Sumayao, et al.; Exp. Physiol. 98, 1505 (2013), Abstract;

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