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Acetyltransferase activity kit

 
ADI-907-026 96 wells 447.00 USD
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  • Universal - suitable for any AcetylCoA-dependent acetyltransferase
  • Robust - Z' factor = 0.87
  • Sensitive - fluorescent detection allows for both DNA and protein assays
  • Higher throughput - homogeneous mix-and-read assay
The Acetyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any acetyl-CoA dependent acetyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
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907-026-Kit-Pathway

Product Specification

Applications:Fluorescent detection, HTS
Activity assay
 
Application Notes:For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store all components at -20°, except positive control at -80°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Black Microtiter plate, Reaction buffer, Assay buffer, Detection solution, Positive control
 
Scientific Background:Acetylation of proteins and drugs is a regulatory event that can result in the activation, inactivation, or detoxification of molecules including histones, kinases, transcription factors, anti-microbial agents, and neurotransmitters. While acetylation can be carried out by a variety of acetyltransferase enzymes using diverse substrates, their common cofactor or acetyl donor is acetyl-CoA.
 

Product Literature References

Structure of Thermoplasma volcanium Ard1 belongs to N-acetyltransferase family member suggesting multiple ligand binding modes with acetyl coenzyme A and coenzyme A: C. Ma, et al.; Biochim. Biophys. Acta 1844, 1790 (2014), Application(s): Acetyltransferase purified from bacteria, Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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