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Methyltransferase activity kit

 
ADI-907-025 96 wells 443.00 USD
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  • Universal - suitable for any AdoMet-dependent methyltransferase
  • Robust - Z factor = 0.83
  • Sensitive - fluorescent detection allows for both DNA and protein assays
  • Higher throughput - homogeneous mix-and-read assay
The Methyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.
907-025-Kit-Pathway
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907-025-Kit-Pathway

Product Specification

Applications:Fluorescent detection, HTS
Activity assay
 
Application Notes:For the measurement of AdoMet-dependent methyltransferase activity in purified in vitro samples that do not contain reductants from any species.
 
Species reactivity:Species independent
 
Use/Stability:Store assay buffer +4° and all other components at -20°.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Black Microtiter plate, Reaction buffer, Assay buffer, Detection solution, Positive control
 
Scientific Background:The methylation of proteins, xenobiotic drugs, nucleic acids, and oligosaccharides is known to regulate important cellular events including meiosis, biosynthesis, development, signal transduction, chromatin remodeling, and gene silencing. The most frequent methyl donor for this type of enzymatic modification is S-adenosyl-L-methionine (AdoMet/SAM). Based on the predicted binding motif for AdoMet, it is estimated that 1% of the mammalian genome encodes a variety of substrate-specific methyltransferases.
 

Product Literature References

Lipotropes protect against pathogen-aggravated stress and mortality in low dose pesticide-exposed fish: N. Kumar, et al.; PLoS One 9, e93499 (2014), Abstract; Full Text
Epigenetic upregulation of Bak by ZBP-89 inhibits the growth of hepatocellular carcinoma: C.G. Ye, et al.; Biochim. Biophys. Acta 1833, 2970 (2013), Abstract; Full Text
Protein kinase C stimulates HuD-mediated mRNA stability and protein expression of neurotrophic factors and enhances dendritic maturation of hippocampal neurons in culture: C.S. Lim, et al.; Hippocampus 22, 2303 (2012), Abstract;

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