Methyltransferase activity kit



ADI-907-025	96 wells	385.00 USD

Universal - suitable for any AdoMet-dependent methyltransferase
Robust - Z factor = 0.83
Sensitive - fluorescent detection allows for both DNA and protein assays
Higher throughput - homogeneous mix-and-read assay

The Methyltransferase activity kit is a homogeneous mix-and-read fluorescent assay for the determination of any S-adenosyl-L-methionine (AdoMet/SAM) dependent methyltransferase activity. It is suitable for end-point or kinetic read options, which is ideal for determining mechanism of action, kinetics, and screening candidate compounds. The assay is amendable to HTS and miniaturization.

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Product Specification

Kit/Set Contains:	Black Microtiter plate, Reaction buffer, Assay buffer, Detection solution, Positive control

Application:	For the measurement of AdoMet-dependent methyltransferase activity in purified in vitro samples that do not contain reductants from any species.

Use/Stability:	Store assay buffer +4° and all other components at -20°.

Miscellaneous/General:	The methylation of proteins, xenobiotic drugs, nucleic acids, and oligosaccharides is known to regulate important cellular events including meiosis, biosynthesis, development, signal transduction, chromatin remodeling, and gene silencing. The most frequent methyl donor for this type of enzymatic modification is S-adenosyl-L-methionine (AdoMet/SAM). Based on the predicted binding motif for AdoMet, it is estimated that 1% of the mammalian genome encodes a variety of substrate-specific methyltransferases.